Rv1484 Enoyl-[acyl-carrier-protein] reductase [NADH] (EC 1.3.1.9)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv1484 inhA Enoyl-[acyl-carrier-protein] reductase [NADH] (EC 1.3.1.9) CDS 1674202 1675011 + 810 269 FALSE

Rv1484 (Enoyl-[acyl-carrier-protein] reductase [NADH] (EC 1.3.1.9)) is predicted to be co-regulated in modules bicluster_0012 with residual 0.58 and bicluster_0477 with residual 0.44.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 3.30 and 0.11 for bicluster_0012 and 0.08 and 0.29 for bicluster_0477 respectively.

These modules are enriched for following go terms: .

This gene is found to be for growth on cholesterol.

Mutant available?:

Product (LegacyBRC) Product (RefSeq)
Enoyl-[acyl-carrier-protein] reductase [NADH] enoyl-(acyl carrier protein) reductase
Operon # Operon
987 - -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Enoyl-[acyl-carrier-protein] reductase (NADH) Fatty acid biosynthesis
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15608622 NP_216000.1 Run
GO:0004318

enoyl-[acyl-carrier-protein] reductase (NADH) activity

enoyl-[acyl-carrier-protein] reductase (NADH) activity

Details: 
Catalysis of the reaction: acyl-[acyl-carrier protein] + NAD+ = trans-2,3-dehydroacyl-[acyl-carrier protein] + NADH + H+.
GO Category: 
molecular_function
1
Total items in this category:  
GO:0004318

enoyl-[acyl-carrier-protein] reductase (NADH) activity

enoyl-[acyl-carrier-protein] reductase (NADH) activity

Details: 
Catalysis of the reaction: acyl-[acyl-carrier protein] + NAD+ = trans-2,3-dehydroacyl-[acyl-carrier protein] + NADH + H+.
GO Category: 
molecular_function
1
Total items in this category:  
GO:0005504

fatty acid binding

fatty acid binding

Details: 
Interacting selectively and non-covalently with fatty acids, aliphatic monocarboxylic acids liberated from naturally occurring fats and oils by hydrolysis.
GO Category: 
molecular_function
2
Total items in this category:  
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
GO:0030497

fatty acid elongation

fatty acid elongation

Details: 
The elongation of a fatty acid chain by the sequential addition of two-carbon units.
GO Category: 
biological_process
4
Total items in this category:  
GO:0070403

NAD+ binding

NAD+ binding

Details: 
Interacting selectively and non-covalently with the oxidized form, NAD, of nicotinamide adenine dinucleotide, a coenzyme involved in many redox and biosynthetic reactions.
GO Category: 
molecular_function
3
Total items in this category:  
GO:0071768

mycolic acid biosynthetic process

mycolic acid biosynthetic process

Details: 
The chemical reactions and pathways resulting in the formation of mycolic acids, beta-hydroxy fatty acids with a long alpha-alkyl side chain.
GO Category: 
biological_process
15
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
NA NA

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: