Rv1547 DNA polymerase III alpha subunit (EC 2.7.7.7)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv1547 dnaE DNA polymerase III alpha subunit (EC 2.7.7.7) CDS 1747694 1751248 + 3 555 1 184 FALSE

Rv1547 (DNA polymerase III alpha subunit (EC 2.7.7.7)) is predicted to be co-regulated in modules bicluster_0268 with residual 0.48 and bicluster_0444 with residual 0.53.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 51.00 and 620.00 for bicluster_0268 and 230.00 and 23,000.00 for bicluster_0444 respectively.

These modules are enriched for following go terms: phosphorylation, cellular respiration, energy derivation by oxidation of organi... cellular component organization, cellular component biogenesis, cellular component organization or bioge....

This gene is found to be for growth on cholesterol.

Mutant available?:

Product (LegacyBRC) Product (RefSeq)
DNA polymerase III subunit alpha DNA polymerase III subunit alpha
Operon # Operon
1030
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

DNA-directed DNA polymerase Purine metabolism, Pyrimidine metabolism
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Purine metabolism

65
Total items in this category:  

KEGG

Pyrimidine metabolism

41
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  

KEGG

DNA replication

14
Total items in this category:  

KEGG

Mismatch repair

19
Total items in this category:  

KEGG

Homologous recombination

22
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15608685 NP_216063.1 Run
GO:0003887

DNA-directed DNA polymerase activity

DNA-directed DNA polymerase activity

Details: 
Catalysis of the reaction: deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1); the synthesis of DNA from deoxyribonucleotide triphosphates in the presence of a DNA template and primer.
GO Category: 
molecular_function
8
Total items in this category:  
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
GO:0040007

growth

growth

Details: 
The increase in size or mass of an entire organism, a part of an organism or a cell.
GO Category: 
biological_process
621
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
NA NA

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: