Rv1594 Quinolinate synthetase (EC 2.5.1.72)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv1594 nadA Quinolinate synthetase (EC 2.5.1.72) CDS 1794756 1795805 + 1 050 349 FALSE

Rv1594 (Quinolinate synthetase (EC 2.5.1.72)) is predicted to be co-regulated in modules bicluster_0428 with residual 0.47 and bicluster_0485 with residual 0.51.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.00 and 0.52 for bicluster_0428 and 0.00 and 0.07 for bicluster_0485 respectively.

These modules are enriched for following go terms: ATPase activity, coupled, ATP-dependent DNA helicase activity, ATPase activity, nuclease activity .

This gene is found to be for growth on cholesterol.

Mutant available?:

Product (LegacyBRC) Product (RefSeq)
Quinolinate synthetase A quinolinate synthetase
Operon # Operon
1051 - -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Nicotinate and nicotinamide metabolism

16
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15608732 NP_216110.1 Run
GO:0008987

quinolinate synthetase A activity

quinolinate synthetase A activity

Details: 
Catalysis of the reaction: iminoaspartate + dihydroxy-acetone-phosphate = quinolinate + 2 H2O + phosphate.
GO Category: 
molecular_function
1
Total items in this category:  
GO:0009435

NAD biosynthetic process

NAD biosynthetic process

Details: 
The chemical reactions and pathways resulting in the formation of nicotinamide adenine dinucleotide, a coenzyme present in most living cells and derived from the B vitamin nicotinic acid; biosynthesis may be of either the oxidized form, NAD, or the reduced form, NADH.
GO Category: 
biological_process
4
Total items in this category:  
GO:0008987

quinolinate synthetase A activity

quinolinate synthetase A activity

Details: 
Catalysis of the reaction: iminoaspartate + dihydroxy-acetone-phosphate = quinolinate + 2 H2O + phosphate.
GO Category: 
molecular_function
1
Total items in this category:  
GO:0040007

growth

growth

Details: 
The increase in size or mass of an entire organism, a part of an organism or a cell.
GO Category: 
biological_process
621
Total items in this category:  
GO:0051539

4 iron, 4 sulfur cluster binding

4 iron, 4 sulfur cluster binding

Details: 
Interacting selectively and non-covalently with a 4 iron, 4 sulfur (4Fe-4S) cluster; this cluster consists of four iron atoms, with the inorganic sulfur atoms found between the irons and acting as bridging ligands.
GO Category: 
molecular_function
5
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
NA NA

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: