Rv1596 Quinolinate phosphoribosyltransferase [decarboxylating] (EC 2.4.2.19)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv1596 nadC Quinolinate phosphoribosyltransferase [decarboxylating] (EC 2.4.2.19) CDS 1797388 1798245 + 858 285 FALSE

Rv1596 (Quinolinate phosphoribosyltransferase [decarboxylating] (EC 2.4.2.19)) is predicted to be co-regulated in modules bicluster_0295 with residual 0.48 and bicluster_0428 with residual 0.47.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 2.70 and 5,200.00 for bicluster_0295 and 0.00 and 0.52 for bicluster_0428 respectively.

These modules are enriched for following go terms: organonitrogen compound biosynthetic pro..., organonitrogen compound metabolic proces..., NAD biosynthetic process, nicotinamide nucleotide biosynthetic pro..., NAD metabolic process, pyridine nucleotide biosynthetic process, acetolactate synthase activity, transferase activity, transferring aldeh... ATPase activity, coupled, ATP-dependent DNA helicase activity, ATPase activity, nuclease activity.

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

Last update: 10/16/2017 - 11:39
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-17929 MT1632 930
Displaying 1 - 1 of 1
Gene Target Differential Expression Distance Expression pvalue Type
No results were found
Displaying 1 - 1 of 1
ChipSeq TF Differential Expression Distance Expression pvalue Type
No results were found
Motif 1 Motif 2 Residual
bicluster_0295
e.value: 
2.7
Motif Bicluster: 
e.value: 
5200
Motif Bicluster: 
0.48
bicluster_0428
e.value: 
0.00000095
Motif Bicluster: 
e.value: 
0.52
Motif Bicluster: 
0.47
Product (LegacyBRC) Product (RefSeq)
Nicotinate-nucleotide pyrophosphorylase [carboxylating] nicotinate-nucleotide pyrophosphorylase
Operon # Operon
1051 - -
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Nicotinate and nicotinamide metabolism

16
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15608734 NP_216112.1 Run
GO:0004514

nicotinate-nucleotide diphosphorylase (carboxylating) activity

nicotinate-nucleotide diphosphorylase (carboxylating) activity

Details: 
Catalysis of the reaction: CO(2) + diphosphate + nicotinate D-ribonucleotide = 5-phospho-alpha-D-ribose 1-diphosphate + 2 H(+) + quinolinate.
GO Category: 
molecular_function
1
Total items in this category:  
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
NA NA

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: