Rv1649 Phenylalanyl-tRNA synthetase alpha chain (EC 6.1.1.20)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv1649 pheS Phenylalanyl-tRNA synthetase alpha chain (EC 6.1.1.20) CDS 1858733 1859758 + 1 026 341 FALSE

Rv1649 (Phenylalanyl-tRNA synthetase alpha chain (EC 6.1.1.20)) is predicted to be co-regulated in modules bicluster_0104 with residual 0.59 and bicluster_0354 with residual 0.44.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 3,600.00 and 3,500.00 for bicluster_0104 and 580.00 and 4,500.00 for bicluster_0354 respectively.

These modules are enriched for following go terms: pyrimidine nucleobase biosynthetic proce..., pyrimidine nucleobase metabolic process, nucleobase biosynthetic process .

This gene is found to be for growth on cholesterol.

Mutant available?:

BASS Score New Primary UTR Primary TSS Re-Annotated Start Tuberculist Annotated Start
-1.278 107 1858593 1858700 1858733
Product (LegacyBRC) Product (RefSeq)
Phenylalanyl-tRNA synthetase alpha chain phenylalanyl-tRNA synthetase subunit alpha
Operon # Operon
1085 -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Phenylalanine--tRNA ligase Aminoacyl-tRNA biosynthesis
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Aminoacyl-tRNA biosynthesis

69
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15608787 NP_216165.1 Run
GO:0004826

phenylalanine-tRNA ligase activity

phenylalanine-tRNA ligase activity

Details: 
Catalysis of the reaction: ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe).
GO Category: 
molecular_function
2
Total items in this category:  
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
GO:0040007

growth

growth

Details: 
The increase in size or mass of an entire organism, a part of an organism or a cell.
GO Category: 
biological_process
621
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
NA NA

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: