Rv1674c Transcriptional regulator, ArsR family

Summary
Product Feature Type Start End Strand Length AA Length is TF
Rv1674c Transcriptional regulator, ArsR family CDS 1899260 1899916 - 657 218 TRUE

Rv1674c (Transcriptional regulator, ArsR family) is predicted to be co-regulated in modules bicluster_0051 with residual 0.43 and bicluster_0207 with residual 0.51.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.00 and 34.00 for bicluster_0051 and 150.00 and 95.00 for bicluster_0207 respectively.

These modules are enriched for following go terms: .

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

Last update: 11/20/2020 - 12:11
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-14745 MT1712 1084
Displaying 1 - 9 of 9
Gene Target Differential Expression Distance Expression pvalue Type
Methyltransferase/methylase
No -149 0.23 0.999978 CDS
PROBABLE CONSERVED TRANSMEMBRANE PROTEIN
No 23 0.22 0.999978 CDS
Transcriptional regulator, ArsR family
Repressed -22 -1.65 0.000000963 CDS
Iron-sulphur-binding reductase
No -24 -0.17 0.999978 Primary.TSS
Transcriptional regulator, ArsR family
Induced 41 5.73 0 CDS
NG,NG-dimethylarginine dimethylaminohydrolase 1 (EC 3.5.3.18)
No -15 -0.83 0.801507 CDS
NG,NG-dimethylarginine dimethylaminohydrolase 1 (EC 3.5.3.18)
No 45 -0.83 0.801507 Internal.TSS
PPE family protein
No 18 -0.09 0.999978 CDS
subtilase family protein
No -58 0.01 0.999978 CDS
Displaying 1 - 4 of 4
ChipSeq TF Differential Expression Distance Expression pvalue Type
HTH-type transcriptional regulator
No 29 -0.26 0.352947 CDS
Histone protein Lsr2
No 29 -0.17 0.639545 CDS
Transcriptional regulator, ArsR family
Induced 41 5.73 0 CDS
transcriptional regulator, ArsR family
Induced 26 0.79 0.0000000021 CDS
Product (LegacyBRC) Product (RefSeq)
PROBABLE TRANSCRIPTIONAL REGULATORY PROTEIN transcriptional regulatory protein
Operon # Operon
1095 -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15608812 NP_216190.1 Run
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
BioProject Accession GEO Series References Repository Sample Method Sample Type
PRJNA254351 GSM1426705 GSE59086 25232098 GEO Tiling Array RNA
PRJNA254351 GSM1426706 GSE59086 25232098 GEO Tiling Array RNA
PRJNA254351 GSM1426707 GSE59086 25232098 GEO Tiling Array RNA
Experiment UCSC Genome Browser
Rv1674c_B456 UCSC Browser Tracks
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.670000 1.09

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated: 0.000916753
p-value INH: 0.812324
Displaying 1 - 17 of 17
Condition Count Day Doublings Fitness U.I Plots
D0U 27 0 0.00 12.53 U
D3I 3 3 3.83 12.21 I
D3U 3 3 3.83 11.90 U
D5I 9 5 6.00 12.53 I
D5U 17 5 6.00 12.25 U
D7I 18 7 8.14 12.58 I
D7U 19 7 8.14 11.67 U
D14I 4 14 15.63 10.33 I
D14U 4 14 15.63 9.85 U
D17I 3 17 19.15 9.94 I
D17U 3 17 19.15 9.17 U
D21I 4 21 23.23 10.11 I
D21U 4 21 23.23 9.41 U
D24I 3 24 26.60 9.45 I
D24U 3 24 26.60 8.82 U
D28I 4 28 30.61 9.48 I
D28U 4 28 30.61 9.01 U