Rv2323c NG,NG-dimethylarginine dimethylaminohydrolase 1 (EC 3.5.3.18)

Summary
Product Feature Type Start End Strand Length AA Length is TF
Rv2323c NG,NG-dimethylarginine dimethylaminohydrolase 1 (EC 3.5.3.18) CDS 2595361 2596269 - 909 302 FALSE

Rv2323c (NG,NG-dimethylarginine dimethylaminohydrolase 1 (EC 3.5.3.18)) is predicted to be co-regulated in modules bicluster_0346 with residual 0.51 and bicluster_0420 with residual 0.47.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.00 and 0.14 for bicluster_0346 and 0.00 and 0.00 for bicluster_0420 respectively.

These modules are enriched for following go terms: .

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

BASS Score Distance to Tuberculist Start Codon Internal TSS New Internal UTR Re-Annotated Start Tuberculist Annotated Start
-0.749 60 2596209 75 2596134 2596269
Last update: 10/16/2017 - 13:07
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-14978 MT2385 392
Product (LegacyBRC) Product (RefSeq)
Putative uncharacterized protein
Operon # Operon
1529 - - - -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15609460 NP_216839.1 Run
GO:0016403

dimethylargininase activity

dimethylargininase activity

Details: 
Catalysis of the reaction: N(G),N(G)-dimethyl-L-arginine + H2O = dimethylamine + L-citrulline.
GO Category: 
molecular_function
1
Total items in this category:  
GO:0016403

dimethylargininase activity

dimethylargininase activity

Details: 
Catalysis of the reaction: N(G),N(G)-dimethyl-L-arginine + H2O = dimethylamine + L-citrulline.
GO Category: 
molecular_function
1
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.070000 1.09

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: