Rv1908c Catalase (EC 1.11.1.6) / Peroxidase (EC 1.11.1.7)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv1908c katG Catalase (EC 1.11.1.6) / Peroxidase (EC 1.11.1.7) CDS 2153889 2156111 - 2 223 740 FALSE

Rv1908c (Catalase (EC 1.11.1.6) / Peroxidase (EC 1.11.1.7)) is predicted to be co-regulated in modules bicluster_0374 with residual 0.50 and bicluster_0470 with residual 0.49.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.01 and 3,200.00 for bicluster_0374 and 150.00 and 8,000.00 for bicluster_0470 respectively.

These modules are enriched for following go terms: .

This gene is found to be for growth on cholesterol.

Mutant available?:

Product (LegacyBRC) Product (RefSeq)
Catalase-peroxidase catalase-peroxidase-peroxynitritase T KATG
Operon # Operon
1251 - -
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Phenylalanine metabolism

13
Total items in this category:  

KEGG

Tryptophan metabolism

47
Total items in this category:  

KEGG

Methane metabolism

26
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  

KEGG

Biosynthesis of secondary metabolites

326
Total items in this category:  

KEGG

Microbial metabolism in diverse environments

296
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15609045 NP_216424.1 Run
GO:0004096

catalase activity

catalase activity

Details: 
Catalysis of the reaction: 2 hydrogen peroxide = O2 + 2 H2O.
GO Category: 
molecular_function
1
Total items in this category:  
GO:0004601

peroxidase activity

peroxidase activity

Details: 
Catalysis of the reaction: donor + hydrogen peroxide = oxidized donor + 2 H2O.
GO Category: 
molecular_function
4
Total items in this category:  
GO:0005576

extracellular region

extracellular region

Details: 
The space external to the outermost structure of a cell. For cells without external protective or external encapsulating structures this refers to space outside of the plasma membrane. This term covers the host cell environment outside an intracellular parasite.
GO Category: 
cellular_component
272
Total items in this category:  
GO:0005829

cytosol

cytosol

Details: 
The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
GO Category: 
cellular_component
371
Total items in this category:  
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
GO:0006979

response to oxidative stress

response to oxidative stress

Details: 
Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of oxidative stress, a state often resulting from exposure to high levels of reactive oxygen species, e.g. superoxide anions, hydrogen peroxide (H2O2), and hydroxyl radicals.
GO Category: 
biological_process
13
Total items in this category:  
GO:0016677

oxidoreductase activity, acting on a heme group of donors, nitrogenous group as acceptor

oxidoreductase activity, acting on a heme group of donors, nitrogenous group as acceptor

Details: 
Catalysis of an oxidation-reduction (redox) reaction in which a heme group acts as a hydrogen or electron donor and reduces a nitrogenous group.
GO Category: 
molecular_function
1
Total items in this category:  
GO:0020037

heme binding

heme binding

Details: 
Interacting selectively and non-covalently with heme, any compound of iron complexed in a porphyrin (tetrapyrrole) ring.
GO Category: 
molecular_function
9
Total items in this category:  
GO:0042744

hydrogen peroxide catabolic process

hydrogen peroxide catabolic process

Details: 
The chemical reactions and pathways resulting in the breakdown of hydrogen peroxide (H2O2).
GO Category: 
biological_process
1
Total items in this category:  
GO:0042803

protein homodimerization activity

protein homodimerization activity

Details: 
Interacting selectively and non-covalently with an identical protein to form a homodimer.
GO Category: 
molecular_function
83
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
NA NA

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: