Rv2027c Histidine kinase response regulator

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv2027c dosT Histidine kinase response regulator CDS 2272787 2274508 - 1 722 573 FALSE

Rv2027c (Histidine kinase response regulator) is predicted to be co-regulated in modules bicluster_0028 with residual 0.52 and bicluster_0196 with residual 0.45.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.00 and 1.50 for bicluster_0028 and 120.00 and 3,000.00 for bicluster_0196 respectively.

These modules are enriched for following go terms: signal transduction, signaling, single organism signaling, cell communication, response to stimulus, signal transducer activity, molecular transducer activity, phosphorelay sensor kinase activity, receptor activity, signaling receptor activity .

This gene is found to be for growth on cholesterol.

Mutant available?:

BASS Score Re-Annotated Start Tuberculist Annotated Start
-0.5 2274463 2274508
Product (LegacyBRC) Product (RefSeq)
Probable histidine kinase response regulator histidine kinase response regulator
Operon # Operon
1331 -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15609164 NP_216543.1 Run
GO:0000287

magnesium ion binding

magnesium ion binding

Details: 
Interacting selectively and non-covalently with magnesium (Mg) ions.
GO Category: 
molecular_function
52
Total items in this category:  
GO:0004672

protein kinase activity

protein kinase activity

Details: 
Catalysis of the phosphorylation of an amino acid residue in a protein, usually according to the reaction: a protein + ATP = a phosphoprotein + ADP.
GO Category: 
molecular_function
18
Total items in this category:  
GO:0004673

protein histidine kinase activity

protein histidine kinase activity

Details: 
Catalysis of the reaction: ATP + protein L-histidine = ADP + protein phospho-L-histidine.
GO Category: 
molecular_function
1
Total items in this category:  
GO:0005509

calcium ion binding

calcium ion binding

Details: 
Interacting selectively and non-covalently with calcium ions (Ca2+).
GO Category: 
molecular_function
3
Total items in this category:  
GO:0005524

ATP binding

ATP binding

Details: 
Interacting selectively and non-covalently with ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
GO Category: 
molecular_function
58
Total items in this category:  
GO:0019825

oxygen binding

oxygen binding

Details: 
Interacting selectively and non-covalently with oxygen (O2).
GO Category: 
molecular_function
4
Total items in this category:  
GO:0019826

oxygen sensor activity

oxygen sensor activity

Details: 
Interacting selectively and non-covalently with and responding, e.g. by conformational change, to changes in the cellular level of oxygen (O2).
GO Category: 
molecular_function
2
Total items in this category:  
GO:0020037

heme binding

heme binding

Details: 
Interacting selectively and non-covalently with heme, any compound of iron complexed in a porphyrin (tetrapyrrole) ring.
GO Category: 
molecular_function
9
Total items in this category:  
GO:0046777

protein autophosphorylation

protein autophosphorylation

Details: 
The phosphorylation by a protein of one or more of its own amino acid residues, or residues on an identical protein.
GO Category: 
biological_process
21
Total items in this category:  
GO:0070025

carbon monoxide binding

carbon monoxide binding

Details: 
Interacting selectively and non-covalently with carbon monoxide (CO).
GO Category: 
molecular_function
3
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.270000 1.01

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: