Rv2031c 14 kDa antigen (16 kDa antigen) (HSP 16.3)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv2031c hspX 14 kDa antigen (16 kDa antigen) (HSP 16.3) CDS 2278498 2278932 - 435 144 FALSE

Rv2031c (14 kDa antigen (16 kDa antigen) (HSP 16.3)) is predicted to be co-regulated in modules bicluster_0182 with residual 0.43 and bicluster_0417 with residual 0.42.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.00 and 0.32 for bicluster_0182 and 0.00 and 0.00 for bicluster_0417 respectively.

These modules are enriched for following go terms: .

This gene is found to be non-essential for growth on cholesterol.

Mutant available?: Yes

Last update: 10/16/2017 - 11:45
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-14725 MT2090 430
Displaying 1 - 1 of 1
Gene Target Differential Expression Distance Expression pvalue Type
No results were found
Displaying 1 - 2 of 2
ChipSeq TF Differential Expression Distance Expression pvalue Type
Transcriptional regulator, XRE family
No 62 -0.3 0.909442 Primary.TSS
HTH-type transcriptional regulator
No 59 -0.26 0.84068 Primary.TSS
Motif 1 Motif 2 Residual
bicluster_0182
e.value: 
2.1e-23
Motif Bicluster: 
e.value: 
0.32
Motif Bicluster: 
0.43
bicluster_0417
e.value: 
3.9e-21
Motif Bicluster: 
e.value: 
0.00018
Motif Bicluster: 
0.42
Product (LegacyBRC) Product (RefSeq)
14 kDa antigen heat shock protein hspX
Operon # Operon
1332 Rv2028c - Rv2029c - Rv2030c - Rv2031c
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15609168 NP_216547.1 Run
GO:0001666

response to hypoxia

response to hypoxia

Details: 
Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a stimulus indicating lowered oxygen tension. Hypoxia, defined as a decline in O2 levels below normoxic levels of 20.8 - 20.95%, results in metabolic adaptation at both the cellular and organismal level.
GO Category: 
biological_process
37
Total items in this category:  
GO:0005618

cell wall

cell wall

Details: 
The rigid or semi-rigid envelope lying outside the cell membrane of plant, fungal, and most prokaryotic cells, maintaining their shape and protecting them from osmotic lysis. In plants it is made of cellulose and, often, lignin; in fungi it is composed largely of polysaccharides; in bacteria it is composed of peptidoglycan.
GO Category: 
cellular_component
320
Total items in this category:  
GO:0005829

cytosol

cytosol

Details: 
The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
GO Category: 
cellular_component
371
Total items in this category:  
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
GO:0006457

protein folding

protein folding

Details: 
The process of assisting in the covalent and noncovalent assembly of single chain polypeptides or multisubunit complexes into the correct tertiary structure.
GO Category: 
biological_process
7
Total items in this category:  
GO:0009267

cellular response to starvation

cellular response to starvation

Details: 
Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of deprivation of nourishment.
GO Category: 
biological_process
9
Total items in this category:  
GO:0009408

response to heat

response to heat

Details: 
Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a heat stimulus, a temperature stimulus above the optimal temperature for that organism.
GO Category: 
biological_process
11
Total items in this category:  
GO:0010039

response to iron ion

response to iron ion

Details: 
Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of an iron ion stimulus.
GO Category: 
biological_process
8
Total items in this category:  
GO:0044121

growth of symbiont in host organelle

growth of symbiont in host organelle

Details: 
The increase in size or mass of a symbiont, occurring in its host's organelle. The host is defined as the larger of the organisms involved in a symbiotic interaction.
GO Category: 
biological_process
5
Total items in this category:  
GO:0044183

protein binding involved in protein folding

protein binding involved in protein folding

Details: 
Interacting selectively and non-covalently with any protein or protein complex (a complex of two or more proteins that may include other nonprotein molecules) that contributes to the process of protein folding.
GO Category: 
molecular_function
2
Total items in this category:  
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
Quantitative Proteomics Data
Cholesterol Essentiality t-test p-value Cholesterol/Glycerol Ratio
non-essential 0.160000 0.64

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.