Rv2029c Tagatose-6-phosphate kinase (EC 2.7.1.144) / 1-phosphofructokinase (EC 2.7.1.56)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv2029c pfkB Tagatose-6-phosphate kinase (EC 2.7.1.144) / 1-phosphofructokinase (EC 2.7.1.56) CDS 2275405 2276424 - 1 020 339 FALSE

Rv2029c (Tagatose-6-phosphate kinase (EC 2.7.1.144) / 1-phosphofructokinase (EC 2.7.1.56)) is predicted to be co-regulated in modules bicluster_0028 with residual 0.52 and bicluster_0404 with residual 0.54.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.00 and 1.50 for bicluster_0028 and 230.00 and 97.00 for bicluster_0404 respectively.

These modules are enriched for following go terms: signal transduction, signaling, single organism signaling, cell communication, response to stimulus, signal transducer activity, molecular transducer activity, phosphorelay sensor kinase activity, receptor activity, signaling receptor activity .

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

Last update: 10/16/2017 - 11:45
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-18046 MT2088 1257
Displaying 1 - 1 of 1
Gene Target Differential Expression Distance Expression pvalue Type
No results were found
Displaying 1 - 1 of 1
ChipSeq TF Differential Expression Distance Expression pvalue Type
No results were found
Motif 1 Motif 2 Residual
bicluster_0028
e.value: 
0.00029
Motif Bicluster: 
e.value: 
1.5
Motif Bicluster: 
0.52
bicluster_0404
e.value: 
230
Motif Bicluster: 
e.value: 
97
Motif Bicluster: 
0.54
Product (LegacyBRC) Product (RefSeq)
Probable phosphofructokinase PfkB [PHOSPHOHEXOKINASE] phosphofructokinase PfkB (phosphohexokinase)
Operon # Operon
1332 - - -
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Glycolysis / Gluconeogenesis

35
Total items in this category:  

KEGG

Pentose phosphate pathway

19
Total items in this category:  

KEGG

Fructose and mannose metabolism

18
Total items in this category:  

KEGG

Galactose metabolism

14
Total items in this category:  

KEGG

Methane metabolism

26
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  

KEGG

Biosynthesis of secondary metabolites

326
Total items in this category:  

KEGG

Microbial metabolism in diverse environments

296
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15609166 NP_216545.1 Run
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.010000 0.67

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: