Rv2045c Probable carboxylesterase LipT (EC 3.1.1.-)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv2045c lipT Probable carboxylesterase LipT (EC 3.1.1.-) CDS 2289685 2291220 - 1 536 511 FALSE

Rv2045c (Probable carboxylesterase LipT (EC 3.1.1.-)) is predicted to be co-regulated in modules bicluster_0028 with residual 0.52 and bicluster_0187 with residual 0.50.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.00 and 1.50 for bicluster_0028 and 0.01 and 0.16 for bicluster_0187 respectively.

These modules are enriched for following go terms: signal transduction, signaling, single organism signaling, cell communication, response to stimulus, signal transducer activity, molecular transducer activity, phosphorelay sensor kinase activity, receptor activity, signaling receptor activity organophosphate biosynthetic process, phospholipid metabolic process, phospholipid biosynthetic process.

This gene is found to be for growth on cholesterol.

Mutant available?:

BASS Score Distance to Tuberculist Start Codon Internal TSS Re-Annotated Start Tuberculist Annotated Start
-1.633 30 2291190 2291190 2291220
Product (LegacyBRC) Product (RefSeq)
Probable carboxylesterase LipT carboxylesterase LipT
Operon # Operon
1338
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15609182 NP_216561.1 Run
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.030000 0.93

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: