Rv2048c Malonyl CoA-acyl carrier protein transacylase (EC 2.3.1.39)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv2048c pks12 Malonyl CoA-acyl carrier protein transacylase (EC 2.3.1.39) CDS 2294531 2306986 - 12 456 4 151 FALSE

Rv2048c (Malonyl CoA-acyl carrier protein transacylase (EC 2.3.1.39)) is predicted to be co-regulated in modules bicluster_0081 with residual 0.55 and bicluster_0474 with residual 0.57.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.53 and 19,000.00 for bicluster_0081 and 0.24 and 18,000.00 for bicluster_0474 respectively.

These modules are enriched for following go terms: .

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

Last update: 10/16/2017 - 11:45
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-13483 MT2108 40
Displaying 1 - 1 of 1
Gene Target Differential Expression Distance Expression pvalue Type
No results were found
Displaying 1 - 1 of 1
ChipSeq TF Differential Expression Distance Expression pvalue Type
No -87 -0.12 0.582538 Primary.TSS
Motif 1 Motif 2 Residual
bicluster_0081
e.value: 
0.53
Motif Bicluster: 
e.value: 
19000
Motif Bicluster: 
0.55
bicluster_0474
e.value: 
0.24
Motif Bicluster: 
e.value: 
18000
Motif Bicluster: 
0.57
Product (LegacyBRC) Product (RefSeq)
Probable polyketide synthase pks12 polyketide synthase pks12
Operon # Operon
1340 -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

[Acyl-carrier-protein] S-malonyltransferase Fatty acid biosynthesis
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15609185 NP_216564.1 Run
GO:0005618

cell wall

cell wall

Details: 
The rigid or semi-rigid envelope lying outside the cell membrane of plant, fungal, and most prokaryotic cells, maintaining their shape and protecting them from osmotic lysis. In plants it is made of cellulose and, often, lignin; in fungi it is composed largely of polysaccharides; in bacteria it is composed of peptidoglycan.
GO Category: 
cellular_component
320
Total items in this category:  
GO:0005829

cytosol

cytosol

Details: 
The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
GO Category: 
cellular_component
371
Total items in this category:  
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
GO:0008610

lipid biosynthetic process

lipid biosynthetic process

Details: 
The chemical reactions and pathways resulting in the formation of lipids, compounds soluble in an organic solvent but not, or sparingly, in an aqueous solvent.
GO Category: 
biological_process
13
Total items in this category:  
GO:0009405

pathogenesis

pathogenesis

Details: 
The set of specific processes that generate the ability of an organism to cause disease in another.
GO Category: 
biological_process
65
Total items in this category:  
GO:0050111

mycocerosate synthase activity

mycocerosate synthase activity

Details: 
Catalysis of the reaction: acyl-CoA + 7n H(+) + n methylmalonyl-CoA + 2n NADPH = n CO(2) + n CoA + n H(2)O + multi-methyl-branched acyl-CoA + 2n NADP(+).
GO Category: 
molecular_function
1
Total items in this category:  
GO:0071766

Actinobacterium-type cell wall biogenesis

Actinobacterium-type cell wall biogenesis

Details: 
A cellular process that results in the biosynthesis of constituent macromolecules, assembly, and arrangement of constituent parts of a cell wall of the type found in Actinobacteria. The cell wall is the rigid or semi-rigid envelope lying outside the cell membrane. Actinobacterial cell walls contain characteristic mycolic acids, of which some are covalently linked to the cell wall peptidoglycan and others accumulate at the cell surface.
GO Category: 
biological_process
19
Total items in this category:  
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.000000 0.15

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: