Rv2054 Dienelactone hydrolase family protein

Summary
Product Feature Type Start End Strand Length AA Length is TF
Rv2054 Dienelactone hydrolase family protein CDS 2313125 2313838 + 714 237 FALSE

Rv2054 (Dienelactone hydrolase family protein) is predicted to be co-regulated in modules bicluster_0364 with residual 0.56 and bicluster_0585 with residual 0.58.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.02 and 2.50 for bicluster_0364 and 0.06 and 350.00 for bicluster_0585 respectively.

These modules are enriched for following go terms: cofactor biosynthetic process, coenzyme metabolic process, cofactor metabolic process.

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

Last update: 10/16/2017 - 11:45
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-18806 MT2114 2616
Displaying 1 - 1 of 1
Gene Target Differential Expression Distance Expression pvalue Type
No results were found
Displaying 1 - 2 of 2
ChipSeq TF Differential Expression Distance Expression pvalue Type
Transcriptional regulator, TetR family
No 17 0.09 0.889833 Primary.TSS
No 62 -0.11 0.999609 Primary.TSS
Motif 1 Motif 2 Residual
bicluster_0364
e.value: 
0.024
Motif Bicluster: 
e.value: 
2.5
Motif Bicluster: 
0.56
bicluster_0585
e.value: 
0.055
Motif Bicluster: 
e.value: 
350
Motif Bicluster: 
0.58
Product (LegacyBRC) Product (RefSeq)
Putative uncharacterized protein
Operon # Operon
1345
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Chlorocyclohexane and chlorobenzene degradation

7
Total items in this category:  

KEGG

Fluorobenzoate degradation

2
Total items in this category:  

KEGG

Toluene degradation

13
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  

KEGG

Microbial metabolism in diverse environments

296
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15609191 NP_216570.1 Run
GO:0005829

cytosol

cytosol

Details: 
The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
GO Category: 
cellular_component
371
Total items in this category:  
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.980000 1.59

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: