Rv2155c UDP-N-acetylmuramoylalanine--D-glutamate ligase (EC 6.3.2.9)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv2155c murD UDP-N-acetylmuramoylalanine--D-glutamate ligase (EC 6.3.2.9) CDS 2414934 2416394 - 1 461 486 FALSE

Rv2155c (UDP-N-acetylmuramoylalanine--D-glutamate ligase (EC 6.3.2.9)) is predicted to be co-regulated in modules bicluster_0288 with residual 0.46 and bicluster_0424 with residual 0.58.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.23 and 2,800.00 for bicluster_0288 and 0.00 and 0.00 for bicluster_0424 respectively.

These modules are enriched for following go terms: cellular component biogenesis, cellular component organization or bioge..., carbohydrate binding, acid-amino acid ligase activity cellular macromolecule catabolic process, nucleobase-containing compound catabolic..., aromatic compound catabolic process, macromolecule catabolic process, exonuclease activity, active with either..., deoxyribonuclease activity, oxidoreductase activity, acting on paire..., exonuclease activity.

This gene is found to be for growth on cholesterol.

Mutant available?:

Displaying 1 - 1 of 1
Gene Target Differential Expression Distance Expression pvalue Type
No results were found
Displaying 1 - 3 of 3
ChipSeq TF Differential Expression Distance Expression pvalue Type
Transcriptional regulator, TetR family
No -26 0.07 0.867868 Internal.TSS
Beta-carotene ketolase (EC 1.14.-.-)
No -48 0.06 0.999473 Internal.TSS
DNA-binding response regulator TrcR
No -37 -0.15 0.710253 Internal.TSS
Motif 1 Motif 2 Residual
bicluster_0288
e.value: 
0.23
Motif Bicluster: 
e.value: 
2800
Motif Bicluster: 
0.46
bicluster_0424
e.value: 
1.4e-35
Motif Bicluster: 
e.value: 
7.6e-26
Motif Bicluster: 
0.58
Product (LegacyBRC) Product (RefSeq)
UDP-N-acetylmuramoylalanine--D-glutamate ligase UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

D-Glutamine and D-glutamate metabolism

3
Total items in this category:  

KEGG

Peptidoglycan biosynthesis

16
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15609292 NP_216671.1 Run
GO:0008150

biological_process

biological_process

Details: 
Any process specifically pertinent to the functioning of integrated living units: cells, tissues, organs, and organisms. A process is a collection of molecular events with a defined beginning and end.
GO Category: 
biological_process
66
Total items in this category:  
GO:0008150

biological_process

biological_process

Details: 
Any process specifically pertinent to the functioning of integrated living units: cells, tissues, organs, and organisms. A process is a collection of molecular events with a defined beginning and end.
GO Category: 
biological_process
66
Total items in this category:  
GO:0008150

biological_process

biological_process

Details: 
Any process specifically pertinent to the functioning of integrated living units: cells, tissues, organs, and organisms. A process is a collection of molecular events with a defined beginning and end.
GO Category: 
biological_process
66
Total items in this category:  
GO:0008150

biological_process

biological_process

Details: 
Any process specifically pertinent to the functioning of integrated living units: cells, tissues, organs, and organisms. A process is a collection of molecular events with a defined beginning and end.
GO Category: 
biological_process
66
Total items in this category:  
GO:0008150

biological_process

biological_process

Details: 
Any process specifically pertinent to the functioning of integrated living units: cells, tissues, organs, and organisms. A process is a collection of molecular events with a defined beginning and end.
GO Category: 
biological_process
66
Total items in this category:  
GO:0008150

biological_process

biological_process

Details: 
Any process specifically pertinent to the functioning of integrated living units: cells, tissues, organs, and organisms. A process is a collection of molecular events with a defined beginning and end.
GO Category: 
biological_process
66
Total items in this category:  
GO:0005515

protein binding

protein binding

Details: 
Interacting selectively and non-covalently with any protein or protein complex (a complex of two or more proteins that may include other nonprotein molecules).
GO Category: 
molecular_function
135
Total items in this category:  
GO:0005829

cytosol

cytosol

Details: 
The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
GO Category: 
cellular_component
371
Total items in this category:  
GO:0040007

growth

growth

Details: 
The increase in size or mass of an entire organism, a part of an organism or a cell.
GO Category: 
biological_process
621
Total items in this category:  
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
NA NA

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: