Rv2157c UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate--D-alanyl-D-alanine ligase (EC 6.3.2.10)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv2157c murF UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate--D-alanyl-D-alanine ligase (EC 6.3.2.10) CDS 2417472 2419004 - 1 533 510 FALSE

Rv2157c (UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate--D-alanyl-D-alanine ligase (EC 6.3.2.10)) is predicted to be co-regulated in modules bicluster_0094 with residual 0.41 and bicluster_0252 with residual 0.55.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 320.00 and 1,400.00 for bicluster_0094 and 930.00 and 3,100.00 for bicluster_0252 respectively.

These modules are enriched for following go terms: carbohydrate derivative metabolic proces..., cell division, cellular component organization, regulation of biological quality regulation of cell shape, regulation of anatomical structure morph..., regulation of cell morphogenesis, regulation of developmental process, cell morphogenesis, anatomical structure morphogenesis, developmental process, cellular component morphogenesis, single-organism developmental process, anatomical structure development, cellular developmental process, regulation of cellular component organiz..., cellular component organization.

This gene is found to be for growth on cholesterol.

Mutant available?:

Displaying 1 - 1 of 1
Gene Target Differential Expression Distance Expression pvalue Type
No results were found
Displaying 1 - 1 of 1
ChipSeq TF Differential Expression Distance Expression pvalue Type
No results were found
Motif 1 Motif 2 Residual
bicluster_0094
e.value: 
320
Motif Bicluster: 
e.value: 
1400
Motif Bicluster: 
0.41
bicluster_0252
e.value: 
930
Motif Bicluster: 
e.value: 
3100
Motif Bicluster: 
0.55
Product (LegacyBRC) Product (RefSeq)
UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase UDP-N-acetylmuramoylalanyl-D-glutamyl-2, 6-diaminopimelate- D-alanyl-D-alanyl ligase MurF
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Lysine biosynthesis

15
Total items in this category:  

KEGG

Peptidoglycan biosynthesis

16
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15609294 NP_216673.1 Run
GO:0047480

UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase activity

UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase activity

Details: 
Catalysis of the reaction: UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysine + ATP + D-alanyl-D-alanine = phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine + ADP.
GO Category: 
molecular_function
1
Total items in this category:  
GO:0008150

biological_process

biological_process

Details: 
Any process specifically pertinent to the functioning of integrated living units: cells, tissues, organs, and organisms. A process is a collection of molecular events with a defined beginning and end.
GO Category: 
biological_process
66
Total items in this category:  
GO:0040007

growth

growth

Details: 
The increase in size or mass of an entire organism, a part of an organism or a cell.
GO Category: 
biological_process
621
Total items in this category:  
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
NA NA

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: