Rv2176 Probable serine/threonine-protein kinase pknL (EC 2.7.11.1)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv2176 pknL Probable serine/threonine-protein kinase pknL (EC 2.7.11.1) CDS 2437941 2439140 + 1 200 399 FALSE

Rv2176 (Probable serine/threonine-protein kinase pknL (EC 2.7.11.1)) is predicted to be co-regulated in modules bicluster_0016 with residual 0.50 and bicluster_0141 with residual 0.53.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 210.00 and 190.00 for bicluster_0016 and 0.31 and 1,600.00 for bicluster_0141 respectively.

These modules are enriched for following go terms: .

This gene is found to be for growth on cholesterol.

Mutant available?:

Product (LegacyBRC) Product (RefSeq)
Probable serine_threonine-protein kinase pknL transmembrane serine/threonine-protein kinase L
Operon # Operon
1424
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15609313 NP_216692.1 Run
GO:0004672

protein kinase activity

protein kinase activity

Details: 
Catalysis of the phosphorylation of an amino acid residue in a protein, usually according to the reaction: a protein + ATP = a phosphoprotein + ADP.
GO Category: 
molecular_function
18
Total items in this category:  
GO:0004674

protein serine/threonine kinase activity

protein serine/threonine kinase activity

Details: 
Catalysis of the reactions: ATP + protein serine = ADP + protein serine phosphate, and ATP + protein threonine = ADP + protein threonine phosphate.
GO Category: 
molecular_function
10
Total items in this category:  
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
GO:0018107

peptidyl-threonine phosphorylation

peptidyl-threonine phosphorylation

Details: 
The phosphorylation of peptidyl-threonine to form peptidyl-O-phospho-L-threonine.
GO Category: 
biological_process
3
Total items in this category:  
GO:0046777

protein autophosphorylation

protein autophosphorylation

Details: 
The phosphorylation by a protein of one or more of its own amino acid residues, or residues on an identical protein.
GO Category: 
biological_process
21
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.240000 1.39

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: