Rv2178c 2-keto-3-deoxy-D-arabino-heptulosonate-7-phosphate synthase II (EC 2.5.1.54)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv2178c aroG 2-keto-3-deoxy-D-arabino-heptulosonate-7-phosphate synthase II (EC 2.5.1.54) CDS 2440332 2441720 - 1 389 462 FALSE

Rv2178c (2-keto-3-deoxy-D-arabino-heptulosonate-7-phosphate synthase II (EC 2.5.1.54)) is predicted to be co-regulated in modules bicluster_0352 with residual 0.50 and bicluster_0513 with residual 0.54.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.01 and 2,900.00 for bicluster_0352 and 130.00 and 270.00 for bicluster_0513 respectively.

These modules are enriched for following go terms: cellular biosynthetic process, organic substance biosynthetic process, translational elongation, macromolecule metabolic process, biosynthetic process, intracellular, macromolecular complex, translation elongation factor activity glutamate metabolic process, dicarboxylic acid biosynthetic process, intracellular protein transport, protein secretion, secretion by cell, secretion, intracellular transport, cellular protein localization, cellular macromolecule localization, protein transmembrane transporter activi..., P-P-bond-hydrolysis-driven protein trans..., macromolecule transmembrane transporter ..., protein transporter activity.

This gene is found to be non-essential for growth on cholesterol.

Mutant available?:

Displaying 1 - 1 of 1
Gene Target Differential Expression Distance Expression pvalue Type
No results were found
Displaying 1 - 1 of 1
ChipSeq TF Differential Expression Distance Expression pvalue Type
No results were found
Motif 1 Motif 2 Residual
bicluster_0352
e.value: 
0.0081
Motif Bicluster: 
e.value: 
2900
Motif Bicluster: 
0.50
bicluster_0513
e.value: 
130
Motif Bicluster: 
e.value: 
270
Motif Bicluster: 
0.54
Product (LegacyBRC) Product (RefSeq)
Probable 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase AroG [DAHP synthetase phenylalanine-repressible] 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase AroG
Operon # Operon
1426 Rv2178c
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Phenylalanine, tyrosine and tryptophan biosynthesis

18
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  

KEGG

Biosynthesis of secondary metabolites

326
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15609315 NP_216694.1 Run
GO:0003849

3-deoxy-7-phosphoheptulonate synthase activity

3-deoxy-7-phosphoheptulonate synthase activity

Details: 
Catalysis of the reaction: D-erythrose 4-phosphate + H(2)O + phosphoenolpyruvate = 7-phospho-2-dehydro-3-deoxy-D-arabino-heptonate + phosphate.
GO Category: 
molecular_function
1
Total items in this category:  
GO:0003849

3-deoxy-7-phosphoheptulonate synthase activity

3-deoxy-7-phosphoheptulonate synthase activity

Details: 
Catalysis of the reaction: D-erythrose 4-phosphate + H(2)O + phosphoenolpyruvate = 7-phospho-2-dehydro-3-deoxy-D-arabino-heptonate + phosphate.
GO Category: 
molecular_function
1
Total items in this category:  
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
GO:0030145

manganese ion binding

manganese ion binding

Details: 
Interacting selectively and non-covalently with manganese (Mn) ions.
GO Category: 
molecular_function
31
Total items in this category:  
GO:0040007

growth

growth

Details: 
The increase in size or mass of an entire organism, a part of an organism or a cell.
GO Category: 
biological_process
621
Total items in this category:  
GO:0051260

protein homooligomerization

protein homooligomerization

Details: 
The process of creating protein oligomers, compounds composed of a small number, usually between three and ten, of identical component monomers. Oligomers may be formed by the polymerization of a number of monomers or the depolymerization of a large protein polymer.
GO Category: 
biological_process
31
Total items in this category:  
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
Quantitative Proteomics Data
Cholesterol Essentiality t-test p-value Cholesterol/Glycerol Ratio
non-essential NA NA

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.