Rv2222c Glutamine synthetase type I (EC 6.3.1.2)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv2222c glnA2 Glutamine synthetase type I (EC 6.3.1.2) CDS 2492402 2493742 - 1 341 446 FALSE

Rv2222c (Glutamine synthetase type I (EC 6.3.1.2)) is predicted to be co-regulated in modules bicluster_0030 with residual 0.60 and bicluster_0140 with residual 0.62.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.05 and 0.81 for bicluster_0030 and 0.00 and 0.00 for bicluster_0140 respectively.

These modules are enriched for following go terms: single-organism metabolic process, single-organism cellular process, biosynthetic process, cellular nitrogen compound metabolic pro..., nitrogen compound metabolic process, membrane part .

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

Last update: 10/16/2017 - 12:06
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-15080 MT2280 1501
Displaying 1 - 1 of 1
Gene Target Differential Expression Distance Expression pvalue Type
No results were found
Displaying 1 - 4 of 4
ChipSeq TF Differential Expression Distance Expression pvalue Type
transcriptional regulator, ArsR family
No -4 -0.23 0.00422931 Primary.TSS
Transcriptional regulator, TetR family
Repressed 8 -0.69 0.000000000711 Primary.TSS
DNA-binding response regulator TrcR
No 1 0 0.998519 Primary.TSS
Transcriptional regulator, ArsR family
Repressed 0 -0.61 0.00000000000000101 Primary.TSS
Motif 1 Motif 2 Residual
bicluster_0030
e.value: 
0.052
Motif Bicluster: 
e.value: 
0.81
Motif Bicluster: 
0.60
bicluster_0140
e.value: 
0.0000061
Motif Bicluster: 
e.value: 
0.000019
Motif Bicluster: 
0.62
Product (LegacyBRC) Product (RefSeq)
Probable glutamine synthetase 2 glutamine synthetase
Operon # Operon
1460
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Alanine, aspartate and glutamate metabolism

26
Total items in this category:  

KEGG

Arginine and proline metabolism

38
Total items in this category:  

KEGG

Nitrogen metabolism

24
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  

KEGG

Microbial metabolism in diverse environments

296
Total items in this category:  

KEGG

Two-component system

53
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15609359 NP_216738.1 Run
GO:0004356

glutamate-ammonia ligase activity

glutamate-ammonia ligase activity

Details: 
Catalysis of the reaction: L-glutamate + ATP + NH(4)(+) = L-glutamine + ADP + 2 H(+) + phosphate.
GO Category: 
molecular_function
4
Total items in this category:  
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
GO:0050001

D-glutaminase activity

D-glutaminase activity

Details: 
Catalysis of the reaction: H2O + L-glutamine = NH3 + D-glutamate.
GO Category: 
molecular_function
1
Total items in this category:  
GO:0051260

protein homooligomerization

protein homooligomerization

Details: 
The process of creating protein oligomers, compounds composed of a small number, usually between three and ten, of identical component monomers. Oligomers may be formed by the polymerization of a number of monomers or the depolymerization of a large protein polymer.
GO Category: 
biological_process
31
Total items in this category:  
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.080000 2.00

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: