Rv2243 Malonyl CoA-acyl carrier protein transacylase (EC 2.3.1.39)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv2243 fabD Malonyl CoA-acyl carrier protein transacylase (EC 2.3.1.39) CDS 2516787 2517695 + 909 302 FALSE

Rv2243 (Malonyl CoA-acyl carrier protein transacylase (EC 2.3.1.39)) is predicted to be co-regulated in modules bicluster_0231 with residual 0.47 and bicluster_0369 with residual 0.51.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 3.10 and 6,500.00 for bicluster_0231 and 5.10 and 230.00 for bicluster_0369 respectively.

These modules are enriched for following go terms: lipid biosynthetic process, lipid metabolic process, transferase activity, methyltransferase activity.

This gene is found to be for growth on cholesterol.

Mutant available?:

Product (LegacyBRC) Product (RefSeq)
Malonyl CoA-acyl carrier protein transacylase acyl-carrier-protein S-malonyltransferase
Operon # Operon
1473
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

[Acyl-carrier-protein] S-malonyltransferase Fatty acid biosynthesis
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Fatty acid biosynthesis

13
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15609380 NP_216759.1 Run
GO:0004314

[acyl-carrier-protein] S-malonyltransferase activity

[acyl-carrier-protein] S-malonyltransferase activity

Details: 
Catalysis of the reaction: malonyl-CoA + [acyl-carrier protein] = CoA + malonyl-[acyl-carrier protein].
GO Category: 
molecular_function
4
Total items in this category:  
GO:0004314

[acyl-carrier-protein] S-malonyltransferase activity

[acyl-carrier-protein] S-malonyltransferase activity

Details: 
Catalysis of the reaction: malonyl-CoA + [acyl-carrier protein] = CoA + malonyl-[acyl-carrier protein].
GO Category: 
molecular_function
4
Total items in this category:  
GO:0005515

protein binding

protein binding

Details: 
Interacting selectively and non-covalently with any protein or protein complex (a complex of two or more proteins that may include other nonprotein molecules).
GO Category: 
molecular_function
135
Total items in this category:  
GO:0006633

fatty acid biosynthetic process

fatty acid biosynthetic process

Details: 
The chemical reactions and pathways resulting in the formation of a fatty acid, any of the aliphatic monocarboxylic acids that can be liberated by hydrolysis from naturally occurring fats and oils. Fatty acids are predominantly straight-chain acids of 4 to 24 carbon atoms, which may be saturated or unsaturated; branched fatty acids and hydroxy fatty acids also occur, and very long chain acids of over 30 carbons are found in waxes.
GO Category: 
biological_process
12
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
NA NA

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: