Rv2243 Malonyl CoA-acyl carrier protein transacylase (EC 2.3.1.39)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv2243 fabD Malonyl CoA-acyl carrier protein transacylase (EC 2.3.1.39) CDS 2516787 2517695 + 909 302 FALSE

Rv2243 (Malonyl CoA-acyl carrier protein transacylase (EC 2.3.1.39)) is predicted to be co-regulated in modules bicluster_0231 with residual 0.47 and bicluster_0369 with residual 0.51.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 3.10 and 6,500.00 for bicluster_0231 and 5.10 and 230.00 for bicluster_0369 respectively.

These modules are enriched for following go terms: lipid biosynthetic process, lipid metabolic process, transferase activity, methyltransferase activity.

This gene is found to be non-essential for growth on cholesterol.

Mutant available?:

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Gene Target Differential Expression Distance Expression pvalue Type
No results were found
Displaying 1 - 10 of 11
ChipSeq TF Differential Expression Distance Expression pvalue Type
Transcriptional regulator, PadR family
No -66 0.03 0.975287 Primary.TSS
Transcriptional regulator, TetR family
No -37 0.22 0.725042 Primary.TSS
Two component DNA binding Transcriptional regulatory protein TcrA
No -49 -0.52 0.414098 Primary.TSS
Possible oxidoreductase subunit
No -44 -0.74 0.0559914 Primary.TSS
Transcriptional regulator, TetR family
No -93 0.38 0.667496 Primary.TSS
Transcriptional regulatory protein
No -67 -0.41 0.26014 Primary.TSS
Transcriptional regulator, AsnC family
No -63 -0.15 0.936281 Primary.TSS
DNA-binding response regulator
Repressed -39 -1.57 0.000000114 Primary.TSS
Histone protein Lsr2
No -61 0.04 0.971632 Primary.TSS
Transcriptional regulator kstR (Rv3574), TetR family
No -45 -0.02 0.972233 Primary.TSS
Motif 1 Motif 2 Residual
bicluster_0231
e.value: 
3.1
Motif Bicluster: 
e.value: 
6500
Motif Bicluster: 
0.47
bicluster_0369
e.value: 
5.1
Motif Bicluster: 
e.value: 
230
Motif Bicluster: 
0.51
Product (LegacyBRC) Product (RefSeq)
Malonyl CoA-acyl carrier protein transacylase acyl-carrier-protein S-malonyltransferase
Operon # Operon
1473 Rv2243
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

[Acyl-carrier-protein] S-malonyltransferase Fatty acid biosynthesis
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Fatty acid biosynthesis

13
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15609380 NP_216759.1 Run
GO:0004314

[acyl-carrier-protein] S-malonyltransferase activity

[acyl-carrier-protein] S-malonyltransferase activity

Details: 
Catalysis of the reaction: malonyl-CoA + [acyl-carrier protein] = CoA + malonyl-[acyl-carrier protein].
GO Category: 
molecular_function
4
Total items in this category:  
GO:0004314

[acyl-carrier-protein] S-malonyltransferase activity

[acyl-carrier-protein] S-malonyltransferase activity

Details: 
Catalysis of the reaction: malonyl-CoA + [acyl-carrier protein] = CoA + malonyl-[acyl-carrier protein].
GO Category: 
molecular_function
4
Total items in this category:  
GO:0005515

protein binding

protein binding

Details: 
Interacting selectively and non-covalently with any protein or protein complex (a complex of two or more proteins that may include other nonprotein molecules).
GO Category: 
molecular_function
135
Total items in this category:  
GO:0006633

fatty acid biosynthetic process

fatty acid biosynthetic process

Details: 
The chemical reactions and pathways resulting in the formation of a fatty acid, any of the aliphatic monocarboxylic acids that can be liberated by hydrolysis from naturally occurring fats and oils. Fatty acids are predominantly straight-chain acids of 4 to 24 carbon atoms, which may be saturated or unsaturated; branched fatty acids and hydroxy fatty acids also occur, and very long chain acids of over 30 carbons are found in waxes.
GO Category: 
biological_process
12
Total items in this category:  
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
Quantitative Proteomics Data
Cholesterol Essentiality t-test p-value Cholesterol/Glycerol Ratio
non-essential NA NA

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.