Rv2252 Diacylglycerol kinase-related protein

Summary
Product Feature Type Start End Strand Length AA Length is TF
Rv2252 Diacylglycerol kinase-related protein CDS 2526989 2527918 + 930 309 FALSE

Rv2252 (Diacylglycerol kinase-related protein) is predicted to be co-regulated in modules bicluster_0107 with residual 0.57 and bicluster_0147 with residual 0.43.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 3.30 and 320.00 for bicluster_0107 and 160.00 and 4,800.00 for bicluster_0147 respectively.

These modules are enriched for following go terms: .

This gene is found to be for growth on cholesterol.

Mutant available?:

Displaying 1 - 1 of 1
Gene Target Differential Expression Distance Expression pvalue Type
No results were found
Displaying 1 - 1 of 1
ChipSeq TF Differential Expression Distance Expression pvalue Type
Transcriptional regulator, IclR family
No -114 0.1 0.932486 CDS
Motif 1 Motif 2 Residual
bicluster_0107
e.value: 
3.3
Motif Bicluster: 
e.value: 
320
Motif Bicluster: 
0.57
bicluster_0147
e.value: 
160
Motif Bicluster: 
e.value: 
4800
Motif Bicluster: 
0.43
Product (LegacyBRC) Product (RefSeq)
Putative uncharacterized protein diacylglycerol kinase
Operon # Operon
1479 - -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15609389 NP_216768.1 Run
GO:0004143

diacylglycerol kinase activity

diacylglycerol kinase activity

Details: 
Catalysis of the reaction: NTP + 1,2-diacylglycerol = NDP + 1,2-diacylglycerol-3-phosphate.
GO Category: 
molecular_function
1
Total items in this category:  
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
GO:0009247

glycolipid biosynthetic process

glycolipid biosynthetic process

Details: 
The chemical reactions and pathways resulting in the formation of glycolipid, a class of 1,2-di-O-acylglycerols joined at oxygen 3 by a glycosidic linkage to a carbohydrate part (usually a mono-, di- or tri-saccharide).
GO Category: 
biological_process
17
Total items in this category:  
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.650000 1.04

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: