Rv2322c Ornithine aminotransferase (EC 2.6.1.13) ; N-terminus domain

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv2322c rocD1 Ornithine aminotransferase (EC 2.6.1.13) ; N-terminus domain CDS 2594699 2595364 - 666 221 FALSE

Rv2322c (Ornithine aminotransferase (EC 2.6.1.13) ; N-terminus domain) is predicted to be co-regulated in modules bicluster_0420 with residual 0.47 and bicluster_0552 with residual 0.57.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.00 and 0.00 for bicluster_0420 and 0.00 and 0.00 for bicluster_0552 respectively.

These modules are enriched for following go terms: IMP biosynthetic process, 'de novo' IMP biosynthetic process, IMP metabolic process.

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

Last update: 10/16/2017 - 13:07
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-14944 MT2384.1 337
Product (LegacyBRC) Product (RefSeq)
PROBABLE ORNITHINE AMINOTRANSFERASE [N-terminus part] ROCD1 [ORNITHINE--OXO-ACID AMINOTRANSFERASE] ornithine aminotransferase
Operon # Operon
1529 - - - -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Ornithine aminotransferase Arginine and proline metabolism
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Arginine and proline metabolism

38
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  

KEGG

Biosynthesis of secondary metabolites

326
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15609459 NP_216838.1 Run
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.750000 1.56

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: