Rv2359 Zinc uptake regulation protein ZUR

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv2359 zur Zinc uptake regulation protein ZUR CDS 2641650 2642042 + 393 130 TRUE

Rv2359 (Zinc uptake regulation protein ZUR) is predicted to be co-regulated in modules bicluster_0335 with residual 0.58 and bicluster_0482 with residual 0.61.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 57.00 and 1,600.00 for bicluster_0335 and 0.02 and 0.93 for bicluster_0482 respectively.

These modules are enriched for following go terms: cellular macromolecule biosynthetic proc..., macromolecule biosynthetic process, macromolecule metabolic process, cytoplasm.

This gene is found to be for growth on cholesterol.

Mutant available?:

Displaying 1 - 6 of 6
Gene Target Differential Expression Distance Expression pvalue Type
Putative metal chaperone, involved in Zn homeostasis, GTPase of COG0523 family
Repressed -38 -0.97 0.0165754 CDS
PPE family protein
Repressed -1 -2.52 0.0000000208 Primary.TSS
No 30 -0.52 0.417813 Primary.TSS
PE family protein
Repressed -63 -1 0.00000199 CDS
Putative uncharacterized protein BCG_3011c
Repressed 23 -2.31 0.00000000000893 Primary.TSS
No -85 -0.16 0.999692 Primary.TSS
Product (LegacyBRC) Product (RefSeq)
PROBABLE FERRIC UPTAKE REGULATION PROTEIN FURB ferric uptake regulation protein FURB
Operon # Operon
1560 -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15609496 NP_216875.1 Run
GO:0003700

sequence-specific DNA binding transcription factor activity

sequence-specific DNA binding transcription factor activity

Details: 
Interacting selectively and non-covalently with a specific DNA sequence in order to modulate transcription. The transcription factor may or may not also interact selectively with a protein or macromolecular complex.
GO Category: 
molecular_function
17
Total items in this category:  
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
GO:0008270

zinc ion binding

zinc ion binding

Details: 
Interacting selectively and non-covalently with zinc (Zn) ions.
GO Category: 
molecular_function
15
Total items in this category:  
GO:0010043

response to zinc ion

response to zinc ion

Details: 
Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a zinc ion stimulus.
GO Category: 
biological_process
2
Total items in this category:  
GO:0042803

protein homodimerization activity

protein homodimerization activity

Details: 
Interacting selectively and non-covalently with an identical protein to form a homodimer.
GO Category: 
molecular_function
83
Total items in this category:  
GO:0045893

positive regulation of transcription, DNA-dependent

positive regulation of transcription, DNA-dependent

Details: 
Any process that activates or increases the frequency, rate or extent of cellular DNA-dependent transcription.
GO Category: 
biological_process
14
Total items in this category:  
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
BioProject Accession GEO Series References Repository Sample Method Sample Type
PRJNA254351 GSM1426819 GSE59086 25232098 GEO Tiling Array RNA
PRJNA254351 GSM1426820 GSE59086 25232098 GEO Tiling Array RNA
PRJNA254351 GSM1426821 GSE59086 25232098 GEO Tiling Array RNA
PRJNA254351 GSM1426822 GSE59086 25232098 GEO Tiling Array RNA
PRJNA254351 GSM1426823 GSE59086 25232098 GEO Tiling Array RNA
Experiment UCSC Genome Browser
Rv2359_B249 UCSC Browser Tracks
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.090000 13.04

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated: 0.000719687
p-value INH: 0.988908
Displaying 1 - 17 of 17
Condition Count Day Doublings Fitness U.I Plots
D0U 27 0 0.00 12.77 U
D3I 3 3 3.83 12.97 I
D3U 3 3 3.83 12.61 U
D5I 9 5 6.00 13.21 I
D5U 17 5 6.00 12.96 U
D7I 18 7 8.14 13.60 I
D7U 19 7 8.14 12.74 U
D14I 4 14 15.63 12.75 I
D14U 4 14 15.63 11.90 U
D17I 3 17 19.15 12.42 I
D17U 3 17 19.15 11.50 U
D21I 4 21 23.23 12.59 I
D21U 4 21 23.23 11.79 U
D24I 3 24 26.60 12.20 I
D24U 3 24 26.60 11.37 U
D28I 4 28 30.61 12.36 I
D28U 4 28 30.61 11.53 U