Rv2377c Protein MbtH

Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv2377c mbtH Protein MbtH CDS 2656215 2656430 - 216 71 FALSE

Rv2377c (Protein MbtH) is predicted to be co-regulated in modules bicluster_0001 with residual 0.40 and bicluster_0094 with residual 0.41.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 740.00 and 1,700.00 for bicluster_0001 and 320.00 and 1,400.00 for bicluster_0094 respectively.

These modules are enriched for following go terms: glycerone kinase activity carbohydrate derivative metabolic proces..., cell division, cellular component organization, regulation of biological quality.

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

Last update: 10/16/2017 - 13:07
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-15129 MT2445.1 1372
Product (LegacyBRC) Product (RefSeq)
Protein mbtH putative protein MbtH
Operon # Operon
1570 - - -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics


Not assigned Not assigned
Locus Tuberculist Genome View


Locus Tag KEGG Pathways


not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network


GI Number Protein ID Blast Conserved Domains
15609514 NP_216893.1 Run
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.420000 10.51

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: