Rv2380c Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv2380c mbtE Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3) CDS 2662067 2667115 - 5 049 1 682 FALSE

Rv2380c (Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3)) is predicted to be co-regulated in modules bicluster_0502 with residual 0.52 and bicluster_0525 with residual 0.52.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.00 and 1.20 for bicluster_0502 and 0.00 and 5.20 for bicluster_0525 respectively.

These modules are enriched for following go terms: .

This gene is found to be non-essential for growth on cholesterol.

Mutant available?:

BASS Score Distance to Tuberculist Start Codon Internal TSS Re-Annotated Start Tuberculist Annotated Start
-1.258 2313 2664802 2667262 2667115
Displaying 1 - 1 of 1
Gene Target Differential Expression Distance Expression pvalue Type
No results were found
Displaying 1 - 1 of 1
ChipSeq TF Differential Expression Distance Expression pvalue Type
Transcriptional regulator, TetR family
No -72 -0.33 0.717274 Internal.TSS
Motif 1 Motif 2 Residual
bicluster_0502
e.value: 
2e-16
Motif Bicluster: 
e.value: 
1.2
Motif Bicluster: 
0.52
bicluster_0525
e.value: 
0.000000000055
Motif Bicluster: 
e.value: 
5.2
Motif Bicluster: 
0.52
Product (LegacyBRC) Product (RefSeq)
PEPTIDE SYNTHETASE MBTE [PEPTIDE SYNTHASE] peptide synthetase MBTE (peptide synthase)
Operon # Operon
1570 Rv2377c - Rv2378c - Rv2379c - Rv2380c
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Long-chain-fatty-acid--CoA ligase Fatty acid metabolism
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Biosynthesis of siderophore group nonribosomal peptides

9
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15609517 NP_216896.1 Run
GO:0000036

ACP phosphopantetheine attachment site binding involved in fatty acid biosynthetic process

ACP phosphopantetheine attachment site binding involved in fatty acid biosynthetic process

Details: 
Interacting selectively with the attachment site of the phosphopantetheine prosthetic group of an acyl carrier protein (ACP) as part of the process of fatty acid biosynthesis.
GO Category: 
molecular_function
5
Total items in this category:  
GO:0005829

cytosol

cytosol

Details: 
The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
GO Category: 
cellular_component
371
Total items in this category:  
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
GO:0031177

phosphopantetheine binding

phosphopantetheine binding

Details: 
Interacting selectively with phosphopantetheine, the vitamin pantetheine 4'-(dihydrogen phosphate).
GO Category: 
molecular_function
5
Total items in this category:  
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
Quantitative Proteomics Data
Cholesterol Essentiality t-test p-value Cholesterol/Glycerol Ratio
non-essential 0.080000 0.83

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.