Rv2386c isochorismate synthase MbtI

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv2386c mbtI isochorismate synthase MbtI CDS 2678653 2680005 - 1 353 450 FALSE

Rv2386c (isochorismate synthase MbtI) is predicted to be co-regulated in modules bicluster_0502 with residual 0.52 and bicluster_0525 with residual 0.52.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.00 and 1.20 for bicluster_0502 and 0.00 and 5.20 for bicluster_0525 respectively.

These modules are enriched for following go terms: .

This gene is found to be for growth on cholesterol.

Mutant available?:

Product (LegacyBRC) Product (RefSeq)
Isochorismate synthase_isochorismate-pyruvate lyase mbtI salicylate synthase MbtI
Operon # Operon
1574
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Biosynthesis of siderophore group nonribosomal peptides

9
Total items in this category:  

KEGG

Biosynthesis of secondary metabolites

326
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
57116981 YP_177877.1 Run
GO:0004049

anthranilate synthase activity

anthranilate synthase activity

Details: 
Catalysis of the reaction: chorismate + L-glutamine = anthranilate + pyruvate + L-glutamate.
GO Category: 
molecular_function
3
Total items in this category:  
GO:0000287

magnesium ion binding

magnesium ion binding

Details: 
Interacting selectively and non-covalently with magnesium (Mg) ions.
GO Category: 
molecular_function
52
Total items in this category:  
GO:0004106

chorismate mutase activity

chorismate mutase activity

Details: 
Catalysis of the reaction: chorismate = prephenate.
GO Category: 
molecular_function
4
Total items in this category:  
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
GO:0008909

isochorismate synthase activity

isochorismate synthase activity

Details: 
Catalysis of the reaction: chorismate = isochorismate.
GO Category: 
molecular_function
2
Total items in this category:  
GO:0009697

salicylic acid biosynthetic process

salicylic acid biosynthetic process

Details: 
The chemical reactions and pathways resulting in the formation of salicylic acid (2-hydroxybenzoic acid), a derivative of benzoic acid.
GO Category: 
biological_process
1
Total items in this category:  
GO:0010106

cellular response to iron ion starvation

cellular response to iron ion starvation

Details: 
Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of deprivation of iron ions.
GO Category: 
biological_process
14
Total items in this category:  
GO:0019290

siderophore biosynthetic process

siderophore biosynthetic process

Details: 
The chemical reactions and pathways resulting in the formation of siderophores, low molecular weight Fe(III)-chelating substances made by aerobic or facultatively anaerobic bacteria, especially when growing under iron deficient conditions. The complexes of Fe(3+)-siderophores have very high stability constants and are taken up by specific transport systems by microorganisms; the subsequent release of iron requires enzymatic action.
GO Category: 
biological_process
6
Total items in this category:  
GO:0040007

growth

growth

Details: 
The increase in size or mass of an entire organism, a part of an organism or a cell.
GO Category: 
biological_process
621
Total items in this category:  
GO:0043904

isochorismate pyruvate lyase activity

isochorismate pyruvate lyase activity

Details: 
Catalysis of the reaction: isochorismate = salicylate + pyruvate.
GO Category: 
molecular_function
1
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.080000 0.27

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: