Rv2391 Ferredoxin--sulfite reductase, actinobacterial type (EC 1.8.7.1)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv2391 nirA Ferredoxin--sulfite reductase, actinobacterial type (EC 1.8.7.1) CDS 2684679 2686370 + 1 692 563 FALSE

Rv2391 (Ferredoxin--sulfite reductase, actinobacterial type (EC 1.8.7.1)) is predicted to be co-regulated in modules bicluster_0119 with residual 0.32 and bicluster_0325 with residual 0.54.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 2,300.00 and 5,600.00 for bicluster_0119 and 0.00 and 72.00 for bicluster_0325 respectively.

These modules are enriched for following go terms: gene expression, macromolecule metabolic process, cellular macromolecule biosynthetic proc..., macromolecule biosynthetic process, ribosome, ribonucleoprotein complex, non-membrane-bounded organelle, intracellular non-membrane-bounded organ..., nucleotidyltransferase activity.

This gene is found to be for growth on cholesterol.

Mutant available?:

BASS Score New Primary UTR Primary TSS Re-Annotated Start Tuberculist Annotated Start
-1.027 216 2684487 2684703 2684679
Product (LegacyBRC) Product (RefSeq)
Sulfite reductase [ferredoxin] ferredoxin-dependent nitrite reductase NIRA
Operon # Operon
1578 - - -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Sulfite reductase (ferredoxin) Sulfur metabolism
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Nitrogen metabolism

24
Total items in this category:  

KEGG

Microbial metabolism in diverse environments

296
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15609528 NP_216907.1 Run
GO:0000103

sulfate assimilation

sulfate assimilation

Details: 
The pathways by which inorganic sulfate is processed and incorporated into sulfated compounds.
GO Category: 
biological_process
2
Total items in this category:  
GO:0016002

sulfite reductase activity

sulfite reductase activity

Details: 
Catalysis of the reaction: hydrogen sulfide + acceptor + 3 H2O = sulfite + reduced acceptor.
GO Category: 
molecular_function
1
Total items in this category:  
GO:0040007

growth

growth

Details: 
The increase in size or mass of an entire organism, a part of an organism or a cell.
GO Category: 
biological_process
621
Total items in this category:  
GO:0051539

4 iron, 4 sulfur cluster binding

4 iron, 4 sulfur cluster binding

Details: 
Interacting selectively and non-covalently with a 4 iron, 4 sulfur (4Fe-4S) cluster; this cluster consists of four iron atoms, with the inorganic sulfur atoms found between the irons and acting as bridging ligands.
GO Category: 
molecular_function
5
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
NA NA

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: