Rv2394 Gamma-glutamyltranspeptidase (EC 2.3.2.2)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv2394 ggtB Gamma-glutamyltranspeptidase (EC 2.3.2.2) CDS 2688010 2689941 + 1 932 643 FALSE

Rv2394 (Gamma-glutamyltranspeptidase (EC 2.3.2.2)) is predicted to be co-regulated in modules bicluster_0104 with residual 0.59 and bicluster_0142 with residual 0.59.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 3,600.00 and 3,500.00 for bicluster_0104 and 420.00 and 7,300.00 for bicluster_0142 respectively.

These modules are enriched for following go terms: pyrimidine nucleobase biosynthetic proce..., pyrimidine nucleobase metabolic process, nucleobase biosynthetic process DNA topological change, glutamate metabolic process, dicarboxylic acid biosynthetic process, DNA conformation change, DNA topoisomerase activity.

This gene is found to be for growth on cholesterol.

Mutant available?:

Displaying 1 - 1 of 1
Gene Target Differential Expression Distance Expression pvalue Type
No results were found
Displaying 1 - 1 of 1
ChipSeq TF Differential Expression Distance Expression pvalue Type
No results were found
Motif 1 Motif 2 Residual
bicluster_0104
e.value: 
3600
Motif Bicluster: 
e.value: 
3500
Motif Bicluster: 
0.59
bicluster_0142
e.value: 
420
Motif Bicluster: 
e.value: 
7300
Motif Bicluster: 
0.59
Product (LegacyBRC) Product (RefSeq)
PROBABLE GAMMA-GLUTAMYLTRANSPEPTIDASE GGTB [GAMMA-GLUTAMYLTRANSFERASE] [GLUTAMYL TRANSPEPTIDASE] gamma-glutamyltranspeptidase precursor GgtB
Operon # Operon
1578 - - -
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Taurine and hypotaurine metabolism

7
Total items in this category:  

KEGG

Selenocompound metabolism

40
Total items in this category:  

KEGG

Cyanoamino acid metabolism

12
Total items in this category:  

KEGG

Glutathione metabolism

14
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15609531 NP_216910.1 Run
GO:0003840

gamma-glutamyltransferase activity

gamma-glutamyltransferase activity

Details: 
Catalysis of the reaction: (5-L-glutamyl)-peptide + an amino acid = peptide + 5-L-glutamyl-amino acid.
GO Category: 
molecular_function
2
Total items in this category:  
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.620000 10.15

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: