Rv2429 Alkylhydroperoxidase protein D

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv2429 ahpD Alkylhydroperoxidase protein D CDS 2726806 2727339 + 534 177 FALSE

Rv2429 (Alkylhydroperoxidase protein D) is predicted to be co-regulated in modules bicluster_0331 with residual 0.41 and bicluster_0485 with residual 0.51.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.00 and 0.00 for bicluster_0331 and 0.00 and 0.07 for bicluster_0485 respectively.

These modules are enriched for following go terms: primary metabolic process, cellular metabolic process, cellular process, organic substance metabolic process, cell, cell part, large ribosomal subunit, small ribosomal subunit .

This gene is found to be for growth on cholesterol.

Mutant available?:

Displaying 1 - 1 of 1
Gene Target Differential Expression Distance Expression pvalue Type
No results were found
Displaying 1 - 1 of 1
ChipSeq TF Differential Expression Distance Expression pvalue Type
No results were found
Motif 1 Motif 2 Residual
bicluster_0331
e.value: 
0.0000000081
Motif Bicluster: 
e.value: 
0.00082
Motif Bicluster: 
0.41
bicluster_0485
e.value: 
0.00012
Motif Bicluster: 
e.value: 
0.068
Motif Bicluster: 
0.51
Product (LegacyBRC) Product (RefSeq)
Rv2429 alkyl hydroperoxide reductase subunit D
Operon # Operon
1601 -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15609566 NP_216945.1 Run
GO:0005515

protein binding

protein binding

Details: 
Interacting selectively and non-covalently with any protein or protein complex (a complex of two or more proteins that may include other nonprotein molecules).
GO Category: 
molecular_function
135
Total items in this category:  
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
GO:0032843

hydroperoxide reductase activity

hydroperoxide reductase activity

Details: 
Catalysis of the reaction: 2 RSH + ROOH = RSSR + ROH + H2O. This reaction is the thiol-dependent conversion of an organic hydroperoxide to the corresponding alcohol.
GO Category: 
molecular_function
2
Total items in this category:  
GO:0042803

protein homodimerization activity

protein homodimerization activity

Details: 
Interacting selectively and non-covalently with an identical protein to form a homodimer.
GO Category: 
molecular_function
83
Total items in this category:  
GO:0045454

cell redox homeostasis

cell redox homeostasis

Details: 
Any process that maintains the redox environment of a cell or compartment within a cell.
GO Category: 
biological_process
9
Total items in this category:  
GO:0070207

protein homotrimerization

protein homotrimerization

Details: 
The formation of a protein homotrimer, a macromolecular structure consisting of three noncovalently associated identical subunits.
GO Category: 
biological_process
6
Total items in this category:  
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.630000 0.90

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: