Rv2455c 2-oxoglutarate oxidoreductase, alpha subunit (EC 1.2.7.3)

Summary
Product Feature Type Start End Strand Length AA Length is TF
Rv2455c 2-oxoglutarate oxidoreductase, alpha subunit (EC 1.2.7.3) CDS 2754743 2756704 - 1 962 653 FALSE

Rv2455c (2-oxoglutarate oxidoreductase, alpha subunit (EC 1.2.7.3)) is predicted to be co-regulated in modules bicluster_0056 with residual 0.52 and bicluster_0369 with residual 0.51.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.11 and 16,000.00 for bicluster_0056 and 5.10 and 230.00 for bicluster_0369 respectively.

These modules are enriched for following go terms: oxidoreductase activity, acting on a sul..., helicase activity, oxidoreductase activity, acting on a sul... lipid biosynthetic process, lipid metabolic process, transferase activity, methyltransferase activity.

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

BASS Score New Primary UTR Primary TSS Re-Annotated Start Tuberculist Annotated Start
-0.421 28 2756747 2756719 2756704
Last update: 10/16/2017 - 13:07
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-15708 MT2530 1552
Product (LegacyBRC) Product (RefSeq)
PROBABLE OXIDOREDUCTASE [ALPHA SUBUNIT] oxidoreductase alpha subunit
Operon # Operon
1618 - - -
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Citrate cycle (TCA cycle)

35
Total items in this category:  

KEGG

Carbon fixation pathways in prokaryotes

42
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  

KEGG

Microbial metabolism in diverse environments

296
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15609592 NP_216971.1 Run
GO:0047553

2-oxoglutarate synthase activity

2-oxoglutarate synthase activity

Details: 
Catalysis of the reaction: 2-oxoglutarate + CoA + oxidized ferredoxin = succinyl-CoA + CO2 + reduced ferredoxin.
GO Category: 
molecular_function
2
Total items in this category:  
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
NA NA

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: