Rv2501c Methylcrotonyl-CoA carboxylase biotin-containing subunit (EC 6.4.1.4)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv2501c accA1 Methylcrotonyl-CoA carboxylase biotin-containing subunit (EC 6.4.1.4) CDS 2814916 2816880 - 1 965 654 FALSE

Rv2501c (Methylcrotonyl-CoA carboxylase biotin-containing subunit (EC 6.4.1.4)) is predicted to be co-regulated in modules bicluster_0240 with residual 0.52 and bicluster_0530 with residual 0.56.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.00 and 5.80 for bicluster_0240 and 0.00 and 0.46 for bicluster_0530 respectively.

These modules are enriched for following go terms: fatty-acyl-CoA synthase activity, C-acyltransferase activity .

This gene is found to be non-essential for growth on cholesterol.

Mutant available?:

Displaying 1 - 1 of 1
Gene Target Differential Expression Distance Expression pvalue Type
No results were found
Displaying 1 - 1 of 1
ChipSeq TF Differential Expression Distance Expression pvalue Type
No results were found
Motif 1 Motif 2 Residual
bicluster_0240
e.value: 
0.00019
Motif Bicluster: 
e.value: 
5.8
Motif Bicluster: 
0.52
bicluster_0530
e.value: 
0.00000008
Motif Bicluster: 
e.value: 
0.46
Motif Bicluster: 
0.56
Product (LegacyBRC) Product (RefSeq)
Acetyl-_propionyl-coenzyme A carboxylase alpha chain acetyl-/propionyl-coenzyme A carboxylase subunit alpha
Operon # Operon
1645 Rv2498c - Rv2499c - Rv2500c - Rv2501c - Rv2502c - Rv2503c - Rv2504c
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Methylcrotonoyl-CoA carboxylase Valine, leucine and isoleucine degradation
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Fatty acid biosynthesis

13
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15609638 NP_217017.1 Run
GO:0004485

methylcrotonoyl-CoA carboxylase activity

methylcrotonoyl-CoA carboxylase activity

Details: 
Catalysis of the reaction: 3-methylbut-2-enoyl-CoA + ATP + bicarbonate = trans-3-methylglutaconyl-CoA + ADP + 2 H(+) + phosphate.
GO Category: 
molecular_function
5
Total items in this category:  
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
Quantitative Proteomics Data
Cholesterol Essentiality t-test p-value Cholesterol/Glycerol Ratio
non-essential 0.300000 1.15

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.