Rv2501c Methylcrotonyl-CoA carboxylase biotin-containing subunit (EC 6.4.1.4)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv2501c accA1 Methylcrotonyl-CoA carboxylase biotin-containing subunit (EC 6.4.1.4) CDS 2814916 2816880 - 1 965 654 FALSE

Rv2501c (Methylcrotonyl-CoA carboxylase biotin-containing subunit (EC 6.4.1.4)) is predicted to be co-regulated in modules bicluster_0240 with residual 0.52 and bicluster_0530 with residual 0.56.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.00 and 5.80 for bicluster_0240 and 0.00 and 0.46 for bicluster_0530 respectively.

These modules are enriched for following go terms: fatty-acyl-CoA synthase activity, C-acyltransferase activity .

This gene is found to be for growth on cholesterol.

Mutant available?:

Product (LegacyBRC) Product (RefSeq)
Acetyl-_propionyl-coenzyme A carboxylase alpha chain acetyl-/propionyl-coenzyme A carboxylase subunit alpha
Operon # Operon
1645 - - - - - -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Methylcrotonoyl-CoA carboxylase Valine, leucine and isoleucine degradation
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Fatty acid biosynthesis

13
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15609638 NP_217017.1 Run
GO:0004485

methylcrotonoyl-CoA carboxylase activity

methylcrotonoyl-CoA carboxylase activity

Details: 
Catalysis of the reaction: 3-methylbut-2-enoyl-CoA + ATP + bicarbonate = trans-3-methylglutaconyl-CoA + ADP + 2 H(+) + phosphate.
GO Category: 
molecular_function
5
Total items in this category:  
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.300000 1.15

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: