Rv2504c Succinyl-CoA:3-ketoacid-coenzyme A transferase subunit A (EC 2.8.3.5)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv2504c scoA Succinyl-CoA:3-ketoacid-coenzyme A transferase subunit A (EC 2.8.3.5) CDS 2819124 2819870 - 747 248 FALSE

Rv2504c (Succinyl-CoA:3-ketoacid-coenzyme A transferase subunit A (EC 2.8.3.5)) is predicted to be co-regulated in modules bicluster_0530 with residual 0.56 and bicluster_0540 with residual 0.52.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.00 and 0.46 for bicluster_0530 and 2,700.00 and 15,000.00 for bicluster_0540 respectively.

These modules are enriched for following go terms: .

This gene is found to be for growth on cholesterol.

Mutant available?:

Product (LegacyBRC) Product (RefSeq)
Probable succinyl-CoA:3-ketoacid-coenzyme A transferase subunit A succinyl-CoA:3-ketoacid-coenzyme A transferase subunit alpha ScoA
Operon # Operon
1645 - - - - - -
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Synthesis and degradation of ketone bodies

12
Total items in this category:  

KEGG

Valine, leucine and isoleucine degradation

60
Total items in this category:  

KEGG

Butanoate metabolism

63
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15609641 NP_217020.1 Run
GO:0008260

3-oxoacid CoA-transferase activity

3-oxoacid CoA-transferase activity

Details: 
Catalysis of the reaction: succinyl-CoA + a 3-oxo acid = succinate + a 3-oxo-acyl-CoA.
GO Category: 
molecular_function
2
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.540000 1.49

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: