Rv2583c GTP pyrophosphokinase (EC 2.7.6.5), (p)ppGpp synthetase I

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv2583c relA GTP pyrophosphokinase (EC 2.7.6.5), (p)ppGpp synthetase I CDS 2907826 2910198 - 2 373 790 FALSE

Rv2583c (GTP pyrophosphokinase (EC 2.7.6.5), (p)ppGpp synthetase I) is predicted to be co-regulated in modules bicluster_0236 with residual 0.53 and bicluster_0482 with residual 0.61.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 1,500.00 and 1,800.00 for bicluster_0236 and 0.02 and 0.93 for bicluster_0482 respectively.

These modules are enriched for following go terms: cellular macromolecule catabolic process, tetrapyrrole metabolic process, tetrapyrrole biosynthetic process, porphyrin-containing compound metabolic ..., porphyrin-containing compound biosynthet... cellular macromolecule biosynthetic proc..., macromolecule biosynthetic process, macromolecule metabolic process, cytoplasm.

This gene is found to be for growth on cholesterol.

Mutant available?:

Product (LegacyBRC) Product (RefSeq)
Probable GTP pyrophosphokinase GTP pyrophosphokinase
Operon # Operon
1697 -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

GTP diphosphokinase Purine metabolism
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Purine metabolism

65
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15609720 NP_217099.1 Run
GO:0005618

cell wall

cell wall

Details: 
The rigid or semi-rigid envelope lying outside the cell membrane of plant, fungal, and most prokaryotic cells, maintaining their shape and protecting them from osmotic lysis. In plants it is made of cellulose and, often, lignin; in fungi it is composed largely of polysaccharides; in bacteria it is composed of peptidoglycan.
GO Category: 
cellular_component
320
Total items in this category:  
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
GO:0008728

GTP diphosphokinase activity

GTP diphosphokinase activity

Details: 
Catalysis of the reaction: ATP + GTP = AMP + guanosine 3'-diphosphate 5'-triphosphate.
GO Category: 
molecular_function
1
Total items in this category:  
GO:0008893

guanosine-3',5'-bis(diphosphate) 3'-diphosphatase activity

guanosine-3',5'-bis(diphosphate) 3'-diphosphatase activity

Details: 
Catalysis of the reaction: guanosine 3',5'-bis(diphosphate) + H2O = guanosine 5'-diphosphate + diphosphate.
GO Category: 
molecular_function
1
Total items in this category:  
GO:0009405

pathogenesis

pathogenesis

Details: 
The set of specific processes that generate the ability of an organism to cause disease in another.
GO Category: 
biological_process
65
Total items in this category:  
GO:0015968

stringent response

stringent response

Details: 
A specific global change in the metabolism of a bacterial cell (the downregulation of nucleic acid and protein synthesis, and the simultaneous upregulation of protein degradation and amino acid synthesis) as a result of starvation.
GO Category: 
biological_process
2
Total items in this category:  
GO:0030145

manganese ion binding

manganese ion binding

Details: 
Interacting selectively and non-covalently with manganese (Mn) ions.
GO Category: 
molecular_function
31
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.240000 0.86

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: