Rv2724c Acyl-CoA dehydrogenase, short-chain specific (EC 1.3.99.2)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv2724c fadE20 Acyl-CoA dehydrogenase, short-chain specific (EC 1.3.99.2) CDS 3036131 3037291 - 1 161 386 FALSE

Rv2724c (Acyl-CoA dehydrogenase, short-chain specific (EC 1.3.99.2)) is predicted to be co-regulated in modules bicluster_0331 with residual 0.41 and bicluster_0475 with residual 0.40.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.00 and 0.00 for bicluster_0331 and 0.00 and 9.10 for bicluster_0475 respectively.

These modules are enriched for following go terms: primary metabolic process, cellular metabolic process, cellular process, organic substance metabolic process, cell, cell part, large ribosomal subunit, small ribosomal subunit fatty acid synthase activity.

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

Last update: 10/16/2017 - 14:17
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-18112 MT2796 1430
Displaying 1 - 1 of 1
Gene Target Differential Expression Distance Expression pvalue Type
No results were found
Displaying 1 - 2 of 2
ChipSeq TF Differential Expression Distance Expression pvalue Type
Mycofactocin system transcriptional regulator
No -33 0.21 0.999588 Primary.TSS
transcriptional regulator, ArsR family
Induced -75 0.83 0.000000015 Primary.TSS
Motif 1 Motif 2 Residual
bicluster_0331
e.value: 
0.0000000081
Motif Bicluster: 
e.value: 
0.00082
Motif Bicluster: 
0.41
bicluster_0475
e.value: 
0.0014
Motif Bicluster: 
e.value: 
9.1
Motif Bicluster: 
0.40
Product (LegacyBRC) Product (RefSeq)
PROBABLE ACYL-CoA DEHYDROGENASE FADE20 acyl-CoA dehydrogenase FADE20
Operon # Operon
1792
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Geraniol degradation

52
Total items in this category:  

KEGG

Naphthalene degradation

40
Total items in this category:  

KEGG

Biosynthesis of secondary metabolites

326
Total items in this category:  

KEGG

Microbial metabolism in diverse environments

296
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15609861 NP_217240.1 Run
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.410000 1.54

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: