Rv2841c Transcription termination protein NusA

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv2841c nusA Transcription termination protein NusA CDS 3148385 3149428 - 1 044 347 FALSE

Rv2841c (Transcription termination protein NusA) is predicted to be co-regulated in modules bicluster_0367 with residual 0.59 and bicluster_0542 with residual 0.60.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.00 and 0.00 for bicluster_0367 and 0.02 and 150.00 for bicluster_0542 respectively.

These modules are enriched for following go terms: protein folding, propionyl-CoA carboxylase activity .

This gene is found to be for growth on cholesterol.

Mutant available?:

Product (LegacyBRC) Product (RefSeq)
Transcription elongation protein nusA transcription elongation factor NusA
Operon # Operon
1861 -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15609978 NP_217357.1 Run
GO:0003723

RNA binding

RNA binding

Details: 
Interacting selectively and non-covalently with an RNA molecule or a portion thereof.
GO Category: 
molecular_function
16
Total items in this category:  
GO:0003676

nucleic acid binding

nucleic acid binding

Details: 
Interacting selectively and non-covalently with any nucleic acid.
GO Category: 
molecular_function
13
Total items in this category:  
GO:0003723

RNA binding

RNA binding

Details: 
Interacting selectively and non-covalently with an RNA molecule or a portion thereof.
GO Category: 
molecular_function
16
Total items in this category:  
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
GO:0030401

transcription antiterminator activity

transcription antiterminator activity

Details: 
OBSOLETE. Functions to prevent the termination of RNA synthesis. Acts as a regulatory device, e.g. in phage lambda, enabling a terminator to be masked from RNA polymerase so that distal genes can be expressed.
GO Category: 
molecular_function
1
Total items in this category:  
GO:0031564

transcription antitermination

transcription antitermination

Details: 
Regulation of transcription by a mechanism that allows RNA polymerase to continue transcription beyond a termination site.
GO Category: 
biological_process
1
Total items in this category:  
GO:0040007

growth

growth

Details: 
The increase in size or mass of an entire organism, a part of an organism or a cell.
GO Category: 
biological_process
621
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
NA NA

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: