Rv2965c Phosphopantetheine adenylyltransferase (EC 2.7.7.3)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv2965c coaD Phosphopantetheine adenylyltransferase (EC 2.7.7.3) CDS 3318330 3318815 - 486 161 FALSE

Rv2965c (Phosphopantetheine adenylyltransferase (EC 2.7.7.3)) is predicted to be co-regulated in modules bicluster_0161 with residual 0.58 and bicluster_0522 with residual 0.62.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 1,500.00 and 11,000.00 for bicluster_0161 and 0.00 and 0.00 for bicluster_0522 respectively.

These modules are enriched for following go terms: purine-containing compound biosynthetic ... cation transmembrane transporter activit....

This gene is found to be for growth on cholesterol.

Mutant available?:

Product (LegacyBRC) Product (RefSeq)
Phosphopantetheine adenylyltransferase phosphopantetheine adenylyltransferase
Operon # Operon
1935
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Pantetheine-phosphate adenylyltransferase Pantothenate and CoA biosynthesis
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Pantothenate and CoA biosynthesis

18
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15610102 NP_217481.1 Run
GO:0004595

pantetheine-phosphate adenylyltransferase activity

pantetheine-phosphate adenylyltransferase activity

Details: 
Catalysis of the reaction: ATP + pantetheine 4'-phosphate = 3'-dephospho-CoA + diphosphate.
GO Category: 
molecular_function
1
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
NA NA

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: