Rv2967c Pyruvate carboxyl transferase (EC 6.4.1.1)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv2967c pca Pyruvate carboxyl transferase (EC 6.4.1.1) CDS 3319663 3323046 - 3 384 1 127 FALSE

Rv2967c (Pyruvate carboxyl transferase (EC 6.4.1.1)) is predicted to be co-regulated in modules bicluster_0161 with residual 0.58 and bicluster_0215 with residual 0.50.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 1,500.00 and 11,000.00 for bicluster_0161 and 13.00 and 140.00 for bicluster_0215 respectively.

These modules are enriched for following go terms: purine-containing compound biosynthetic ... .

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

Last update: 10/16/2017 - 14:41
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-15722 MT3045 2242
Displaying 1 - 1 of 1
Gene Target Differential Expression Distance Expression pvalue Type
No results were found
Displaying 1 - 1 of 1
ChipSeq TF Differential Expression Distance Expression pvalue Type
Transcriptional regulatory protein
Repressed -38 -1.35 3.83e-25 CDS
Motif 1 Motif 2 Residual
bicluster_0161
e.value: 
1500
Motif Bicluster: 
e.value: 
11000
Motif Bicluster: 
0.58
bicluster_0215
e.value: 
13
Motif Bicluster: 
e.value: 
140
Motif Bicluster: 
0.50
Product (LegacyBRC) Product (RefSeq)
PROBABLE PYRUVATE CARBOXYLASE PCA [PYRUVIC CARBOXYLASE] pyruvate carboxylase
Operon # Operon
1937 - -
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Citrate cycle (TCA cycle)

35
Total items in this category:  

KEGG

Pyruvate metabolism

40
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  

KEGG

Microbial metabolism in diverse environments

296
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15610104 NP_217483.1 Run
GO:0004736

pyruvate carboxylase activity

pyruvate carboxylase activity

Details: 
Catalysis of the reaction: ATP + bicarbonate + pyruvate = ADP + 2 H(+) + oxaloacetate + phosphate.
GO Category: 
molecular_function
1
Total items in this category:  
GO:0005618

cell wall

cell wall

Details: 
The rigid or semi-rigid envelope lying outside the cell membrane of plant, fungal, and most prokaryotic cells, maintaining their shape and protecting them from osmotic lysis. In plants it is made of cellulose and, often, lignin; in fungi it is composed largely of polysaccharides; in bacteria it is composed of peptidoglycan.
GO Category: 
cellular_component
320
Total items in this category:  
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
GO:0040007

growth

growth

Details: 
The increase in size or mass of an entire organism, a part of an organism or a cell.
GO Category: 
biological_process
621
Total items in this category:  
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.690000 1.28

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: