Rv3048c Ribonucleotide reductase of class Ib (aerobic), beta subunit (EC 1.17.4.1)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv3048c nrdF2 Ribonucleotide reductase of class Ib (aerobic), beta subunit (EC 1.17.4.1) CDS 3408404 3409378 - 975 324 FALSE

Rv3048c (Ribonucleotide reductase of class Ib (aerobic), beta subunit (EC 1.17.4.1)) is predicted to be co-regulated in modules bicluster_0263 with residual 0.52 and bicluster_0486 with residual 0.55.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 940.00 and 17.00 for bicluster_0263 and 500.00 and 840.00 for bicluster_0486 respectively.

These modules are enriched for following go terms: DNA binding, nucleic acid binding.

This gene is found to be for growth on cholesterol.

Mutant available?:

Product (LegacyBRC) Product (RefSeq)
RIBONUCLEOSIDE-DIPHOSPHATE REDUCTASE [BETA CHAIN] NRDF2 [RIBONUCLEOTIDE REDUCTASE SMALL SUBUNIT] [R2F PROTEIN] ribonucleotide-diphosphate reductase subunit beta
Operon # Operon
1994
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Purine metabolism

65
Total items in this category:  

KEGG

Pyrimidine metabolism

41
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
57117051 YP_177921.1 Run
GO:0004748

ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor

ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor

Details: 
Catalysis of the reaction: 2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin. Thioredoxin disulfide is the oxidized form of thioredoxin.
GO Category: 
molecular_function
4
Total items in this category:  
GO:0005515

protein binding

protein binding

Details: 
Interacting selectively and non-covalently with any protein or protein complex (a complex of two or more proteins that may include other nonprotein molecules).
GO Category: 
molecular_function
135
Total items in this category:  
GO:0005971

ribonucleoside-diphosphate reductase complex

ribonucleoside-diphosphate reductase complex

Details: 
An enzyme complex composed of 2-4 or more subunits, which usually contains nonheme iron and requires ATP for catalysis. Catalyzes the formation of 2'-deoxyribonucleoside diphosphate from ribonucleoside diphosphate, using either thioredoxin disulfide or glutaredoxin disulfide as an acceptor.
GO Category: 
cellular_component
2
Total items in this category:  
GO:0009263

deoxyribonucleotide biosynthetic process

deoxyribonucleotide biosynthetic process

Details: 
The chemical reactions and pathways resulting in the formation of a deoxyribonucleotide, a compound consisting of deoxyribonucleoside (a base linked to a deoxyribose sugar) esterified with a phosphate group at either the 3' or 5'-hydroxyl group of the sugar.
GO Category: 
biological_process
2
Total items in this category:  
GO:0040007

growth

growth

Details: 
The increase in size or mass of an entire organism, a part of an organism or a cell.
GO Category: 
biological_process
621
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
NA NA

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: