Rv3149 NADH-ubiquinone oxidoreductase chain E (EC 1.6.5.3)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv3149 nuoE NADH-ubiquinone oxidoreductase chain E (EC 1.6.5.3) CDS 3514657 3515415 + 759 252 FALSE

Rv3149 (NADH-ubiquinone oxidoreductase chain E (EC 1.6.5.3)) is predicted to be co-regulated in modules bicluster_0165 with residual 0.44 and bicluster_0268 with residual 0.48.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.03 and 0.07 for bicluster_0165 and 51.00 and 620.00 for bicluster_0268 respectively.

These modules are enriched for following go terms: phosphate-containing compound metabolic ..., phosphorus metabolic process, NAD binding phosphorylation, cellular respiration, energy derivation by oxidation of organi....

This gene is found to be for growth on cholesterol.

Mutant available?:

Product (LegacyBRC) Product (RefSeq)
NADH-quinone oxidoreductase subunit E NADH dehydrogenase subunit E
Operon # Operon
2057 - - - - - -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

NADH dehydrogenase (ubiquinone) Oxidative phosphorylation
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Oxidative phosphorylation

47
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15610285 NP_217665.1 Run
GO:0008137

NADH dehydrogenase (ubiquinone) activity

NADH dehydrogenase (ubiquinone) activity

Details: 
Catalysis of the reaction: NADH + H+ + ubiquinone = NAD+ + ubiquinol.
GO Category: 
molecular_function
15
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.320000 0.43

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: