Rv3151 NADH-ubiquinone oxidoreductase chain G (EC 1.6.5.3)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv3151 nuoG NADH-ubiquinone oxidoreductase chain G (EC 1.6.5.3) CDS 3516746 3519166 + 2 421 806 FALSE

Rv3151 (NADH-ubiquinone oxidoreductase chain G (EC 1.6.5.3)) is predicted to be co-regulated in modules bicluster_0165 with residual 0.44 and bicluster_0268 with residual 0.48.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.03 and 0.07 for bicluster_0165 and 51.00 and 620.00 for bicluster_0268 respectively.

These modules are enriched for following go terms: phosphate-containing compound metabolic ..., phosphorus metabolic process, NAD binding phosphorylation, cellular respiration, energy derivation by oxidation of organi....

This gene is found to be for growth on cholesterol.

Mutant available?:

Product (LegacyBRC) Product (RefSeq)
NADH-quinone oxidoreductase subunit G NADH dehydrogenase subunit G
Operon # Operon
2057 - - - - - -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

NADH dehydrogenase (ubiquinone) Oxidative phosphorylation
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Oxidative phosphorylation

47
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15610287 NP_217667.1 Run
GO:0008137

NADH dehydrogenase (ubiquinone) activity

NADH dehydrogenase (ubiquinone) activity

Details: 
Catalysis of the reaction: NADH + H+ + ubiquinone = NAD+ + ubiquinol.
GO Category: 
molecular_function
15
Total items in this category:  
GO:0005618

cell wall

cell wall

Details: 
The rigid or semi-rigid envelope lying outside the cell membrane of plant, fungal, and most prokaryotic cells, maintaining their shape and protecting them from osmotic lysis. In plants it is made of cellulose and, often, lignin; in fungi it is composed largely of polysaccharides; in bacteria it is composed of peptidoglycan.
GO Category: 
cellular_component
320
Total items in this category:  
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
GO:0009405

pathogenesis

pathogenesis

Details: 
The set of specific processes that generate the ability of an organism to cause disease in another.
GO Category: 
biological_process
65
Total items in this category:  
GO:0033668

negative regulation by symbiont of host apoptotic process

negative regulation by symbiont of host apoptotic process

Details: 
Any process in which an organism stops, prevents, or reduces the frequency, rate or extent of programmed cell death in the host, where programmed cell death proceeds by apoptosis. The host is defined as the larger of the organisms involved in a symbiotic interaction.
GO Category: 
biological_process
1
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.680000 0.84

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: