Rv3232c UDP-galactose-lipid carrier transferase (EC 2.-.-.-)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv3232c pvdS UDP-galactose-lipid carrier transferase (EC 2.-.-.-) CDS 3608870 3609757 - 888 295 FALSE

Rv3232c (UDP-galactose-lipid carrier transferase (EC 2.-.-.-)) is predicted to be co-regulated in modules bicluster_0068 with residual 0.53 and bicluster_0532 with residual 0.51.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 20.00 and 470.00 for bicluster_0068 and 0.00 and 0.05 for bicluster_0532 respectively.

These modules are enriched for following go terms: methyltransferase activity, transferase activity, transferring one-c... .

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

BASS Score Distance to Tuberculist Start Codon Internal TSS New Internal UTR New Primary UTR Primary TSS Re-Annotated Start Tuberculist Annotated Start
-0.324 19 3609738 2 233 3609969 3609736 3609757
Last update: 10/16/2017 - 15:27
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-13628 MT3329 485
Product (LegacyBRC) Product (RefSeq)
POSSIBLE TRANSCRIPTIONAL REGULATORY PROTEIN PVDS [PROBABLE RNA POLYMERASE SIGMA FACTOR] transcriptional regulatory protein PvdS
Operon # Operon
2116 - - -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Transferases. Puromycin biosynthesis, Lipopolysaccharide biosynthesis
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15610368 NP_217749.1 Run
GO:0005515

protein binding

protein binding

Details: 
Interacting selectively and non-covalently with any protein or protein complex (a complex of two or more proteins that may include other nonprotein molecules).
GO Category: 
molecular_function
135
Total items in this category:  
GO:0005525

GTP binding

GTP binding

Details: 
Interacting selectively and non-covalently with GTP, guanosine triphosphate.
GO Category: 
molecular_function
18
Total items in this category:  
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
GO:0006183

GTP biosynthetic process

GTP biosynthetic process

Details: 
The chemical reactions and pathways resulting in the formation of GTP, guanosine triphosphate.
GO Category: 
biological_process
1
Total items in this category:  
GO:0008976

polyphosphate kinase activity

polyphosphate kinase activity

Details: 
Catalysis of the reaction: ATP + phosphate(n) = ADP + phosphate(n+1).
GO Category: 
molecular_function
2
Total items in this category:  
GO:0030808

regulation of nucleotide biosynthetic process

regulation of nucleotide biosynthetic process

Details: 
Any process that modulates the frequency, rate or extent of the chemical reactions and pathways resulting in the formation of nucleotides.
GO Category: 
biological_process
1
Total items in this category:  
GO:0044121

growth of symbiont in host organelle

growth of symbiont in host organelle

Details: 
The increase in size or mass of a symbiont, occurring in its host's organelle. The host is defined as the larger of the organisms involved in a symbiotic interaction.
GO Category: 
biological_process
5
Total items in this category:  
GO:0046777

protein autophosphorylation

protein autophosphorylation

Details: 
The phosphorylation by a protein of one or more of its own amino acid residues, or residues on an identical protein.
GO Category: 
biological_process
21
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.440000 1.15

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: