Rv3234c Wax ester synthase/acyl-CoA:diacylglycerol acyltransferase

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv3234c tgs3 Wax ester synthase/acyl-CoA:diacylglycerol acyltransferase CDS 3610374 3611189 - 816 271 FALSE

Rv3234c (Wax ester synthase/acyl-CoA:diacylglycerol acyltransferase) is predicted to be co-regulated in modules bicluster_0212 with residual 0.55 and bicluster_0532 with residual 0.51.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.00 and 0.00 for bicluster_0212 and 0.00 and 0.05 for bicluster_0532 respectively.

These modules are enriched for following go terms: .

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

Last update: 10/16/2017 - 15:27
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-17820 MT3331 137
Product (LegacyBRC) Product (RefSeq)
UPF0089 protein Rv3234c_MT3331
Operon # Operon
2116 - - -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15610370 NP_217751.1 Run
GO:0001666

response to hypoxia

response to hypoxia

Details: 
Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a stimulus indicating lowered oxygen tension. Hypoxia, defined as a decline in O2 levels below normoxic levels of 20.8 - 20.95%, results in metabolic adaptation at both the cellular and organismal level.
GO Category: 
biological_process
37
Total items in this category:  
GO:0004144

diacylglycerol O-acyltransferase activity

diacylglycerol O-acyltransferase activity

Details: 
Catalysis of the reaction: acyl-CoA + 1,2-diacylglycerol = CoA + triacylglycerol.
GO Category: 
molecular_function
6
Total items in this category:  
GO:0045017

glycerolipid biosynthetic process

glycerolipid biosynthetic process

Details: 
The chemical reactions and pathways resulting in the formation of glycerolipids, any lipid with a glycerol backbone.
GO Category: 
biological_process
7
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.830000 1.40

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: