Rv3275c Phosphoribosylaminoimidazole carboxylase catalytic subunit (EC 4.1.1.21)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv3275c purE Phosphoribosylaminoimidazole carboxylase catalytic subunit (EC 4.1.1.21) CDS 3658114 3658638 - 525 174 FALSE

Rv3275c (Phosphoribosylaminoimidazole carboxylase catalytic subunit (EC 4.1.1.21)) is predicted to be co-regulated in modules bicluster_0554 with residual 0.55 and bicluster_0581 with residual 0.61.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 8.40 and 31.00 for bicluster_0554 and 0.01 and 120.00 for bicluster_0581 respectively.

These modules are enriched for following go terms: cellular amide metabolic process, amide biosynthetic process .

This gene is found to be for growth on cholesterol.

Mutant available?:

Displaying 1 - 1 of 1
Gene Target Differential Expression Distance Expression pvalue Type
No results were found
Displaying 1 - 1 of 1
ChipSeq TF Differential Expression Distance Expression pvalue Type
Transcriptional regulator, MarR family
No 63 0.01 0.977974 Primary.TSS
Motif 1 Motif 2 Residual
bicluster_0554
e.value: 
8.4
Motif Bicluster: 
e.value: 
31
Motif Bicluster: 
0.55
bicluster_0581
e.value: 
0.0065
Motif Bicluster: 
e.value: 
120
Motif Bicluster: 
0.61
Product (LegacyBRC) Product (RefSeq)
Phosphoribosylaminoimidazole carboxylase catalytic subunit phosphoribosylaminoimidazole carboxylase catalytic subunit
Operon # Operon
2142 - -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Phosphoribosylaminoimidazole carboxylase Purine metabolism
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Purine metabolism

65
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  

KEGG

Biosynthesis of secondary metabolites

326
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15610411 NP_217792.1 Run
GO:0004638

phosphoribosylaminoimidazole carboxylase activity

phosphoribosylaminoimidazole carboxylase activity

Details: 
Catalysis of the reaction: 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate + 2 H(+) = 5-amino-1-(5-phospho-D-ribosyl)imidazole + CO(2).
GO Category: 
molecular_function
2
Total items in this category:  
GO:0040007

growth

growth

Details: 
The increase in size or mass of an entire organism, a part of an organism or a cell.
GO Category: 
biological_process
621
Total items in this category:  
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
NA NA

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: