Rv3276c Phosphoribosylaminoimidazole carboxylase ATPase subunit (EC 4.1.1.21)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv3276c purK Phosphoribosylaminoimidazole carboxylase ATPase subunit (EC 4.1.1.21) CDS 3658635 3659924 - 1 290 429 FALSE

Rv3276c (Phosphoribosylaminoimidazole carboxylase ATPase subunit (EC 4.1.1.21)) is predicted to be co-regulated in modules bicluster_0554 with residual 0.55 and bicluster_0581 with residual 0.61.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 8.40 and 31.00 for bicluster_0554 and 0.01 and 120.00 for bicluster_0581 respectively.

These modules are enriched for following go terms: cellular amide metabolic process, amide biosynthetic process .

This gene is found to be for growth on cholesterol.

Mutant available?:

BASS Score Distance to Tuberculist Start Codon Internal TSS New Internal UTR Re-Annotated Start Tuberculist Annotated Start
-0.377 9 3659915 54 3659861 3659924
Displaying 1 - 1 of 1
Gene Target Differential Expression Distance Expression pvalue Type
No results were found
Displaying 1 - 5 of 5
ChipSeq TF Differential Expression Distance Expression pvalue Type
No 25 -0.39 0.00393239 CDS
DNA-binding response regulator mtrA
No 68 -0.14 0.649446 CDS
Transcriptional regulator, MarR family
No 10 -0.15 0.291301 Internal.TSS
Transcriptional regulator, MarR family
No 63 -0.15 0.291301 Internal.TSS
No 34 -0.39 0.00393239 Internal.TSS
Motif 1 Motif 2 Residual
bicluster_0554
e.value: 
8.4
Motif Bicluster: 
e.value: 
31
Motif Bicluster: 
0.55
bicluster_0581
e.value: 
0.0065
Motif Bicluster: 
e.value: 
120
Motif Bicluster: 
0.61
Product (LegacyBRC) Product (RefSeq)
Phosphoribosylaminoimidazole carboxylase ATPase subunit phosphoribosylaminoimidazole carboxylase ATPase subunit
Operon # Operon
2142 - -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Phosphoribosylaminoimidazole carboxylase Purine metabolism
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Purine metabolism

65
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  

KEGG

Biosynthesis of secondary metabolites

326
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15610412 NP_217793.1 Run
GO:0004638

phosphoribosylaminoimidazole carboxylase activity

phosphoribosylaminoimidazole carboxylase activity

Details: 
Catalysis of the reaction: 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate + 2 H(+) = 5-amino-1-(5-phospho-D-ribosyl)imidazole + CO(2).
GO Category: 
molecular_function
2
Total items in this category:  
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
NA NA

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: