Rv3299c Arylsulfatase (EC 3.1.6.1)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv3299c atsB Arylsulfatase (EC 3.1.6.1) CDS 3683051 3685963 - 2 913 970 FALSE

Rv3299c (Arylsulfatase (EC 3.1.6.1)) is predicted to be co-regulated in modules bicluster_0230 with residual 0.33 and bicluster_0332 with residual 0.53.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 2,600.00 and 2,200.00 for bicluster_0230 and 0.02 and 590.00 for bicluster_0332 respectively.

These modules are enriched for following go terms: purine nucleotide biosynthetic process, purine nucleotide metabolic process, purine-containing compound biosynthetic ....

This gene is found to be for growth on cholesterol.

Mutant available?:

Displaying 1 - 1 of 1
Gene Target Differential Expression Distance Expression pvalue Type
No results were found
Displaying 1 - 1 of 1
ChipSeq TF Differential Expression Distance Expression pvalue Type
No -57 0.26 0.150674 Primary.TSS
Motif 1 Motif 2 Residual
bicluster_0230
e.value: 
2600
Motif Bicluster: 
e.value: 
2200
Motif Bicluster: 
0.33
bicluster_0332
e.value: 
0.02
Motif Bicluster: 
e.value: 
590
Motif Bicluster: 
0.53
Product (LegacyBRC) Product (RefSeq)
PROBABLE ARYLSULFATASE ATSB [ARYL-SULFATE SULPHOHYDROLASE] [SULFATASE] arylsulfatase AtsB
Operon # Operon
2156 - - -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Arylsulfatase C21-Steroid hormone metabolism, Sphingolipid metabolism
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15610435 NP_217816.1 Run
GO:0005829

cytosol

cytosol

Details: 
The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
GO Category: 
cellular_component
371
Total items in this category:  
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.190000 1.33

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: