Rv3300c Ribosomal large subunit pseudouridine synthase A (EC 4.2.1.70)

Summary
Product Feature Type Start End Strand Length AA Length is TF
Rv3300c Ribosomal large subunit pseudouridine synthase A (EC 4.2.1.70) CDS 3685983 3686900 - 918 305 FALSE

Rv3300c (Ribosomal large subunit pseudouridine synthase A (EC 4.2.1.70)) is predicted to be co-regulated in modules bicluster_0268 with residual 0.48 and bicluster_0490 with residual 0.57.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 51.00 and 620.00 for bicluster_0268 and 0.01 and 730.00 for bicluster_0490 respectively.

These modules are enriched for following go terms: phosphorylation, cellular respiration, energy derivation by oxidation of organi... .

This gene is found to be for growth on cholesterol.

Mutant available?:

BASS Score Distance to Tuberculist Start Codon Internal TSS Re-Annotated Start Tuberculist Annotated Start
-0.68 887 3686013 3686867 3686900
Product (LegacyBRC) Product (RefSeq)
Pseudouridine synthase
Operon # Operon
2156 - - -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Pseudouridylate synthase Pyrimidine metabolism
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15610436 NP_217817.1 Run
GO:0004730

pseudouridylate synthase activity

pseudouridylate synthase activity

Details: 
Catalysis of the reaction: D-ribose 5-phosphate + uracil = H(2)O + pseudouridine 5'-phosphate.
GO Category: 
molecular_function
2
Total items in this category:  
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.170000 0.58

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: