Rv3307 Purine nucleoside phosphorylase (EC 2.4.2.1)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv3307 deoD Purine nucleoside phosphorylase (EC 2.4.2.1) CDS 3694054 3694860 + 807 268 FALSE

Rv3307 (Purine nucleoside phosphorylase (EC 2.4.2.1)) is predicted to be co-regulated in modules bicluster_0533 with residual 0.51 and bicluster_0571 with residual 0.32.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 12.00 and 60.00 for bicluster_0533 and 1,500.00 and 3,500.00 for bicluster_0571 respectively.

These modules are enriched for following go terms: phosphate-containing compound metabolic ..., phosphorus metabolic process, organophosphate biosynthetic process, coenzyme biosynthetic process, cofactor biosynthetic process .

This gene is found to be for growth on cholesterol.

Mutant available?:

Displaying 1 - 1 of 1
Gene Target Differential Expression Distance Expression pvalue Type
No results were found
Displaying 1 - 1 of 1
ChipSeq TF Differential Expression Distance Expression pvalue Type
No results were found
Motif 1 Motif 2 Residual
bicluster_0533
e.value: 
12
Motif Bicluster: 
e.value: 
60
Motif Bicluster: 
0.51
bicluster_0571
e.value: 
1500
Motif Bicluster: 
e.value: 
3500
Motif Bicluster: 
0.32
Product (LegacyBRC) Product (RefSeq)
Rv3307 purine nucleoside phosphorylase
Operon # Operon
2160 -
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Purine metabolism

65
Total items in this category:  

KEGG

Pyrimidine metabolism

41
Total items in this category:  

KEGG

Nicotinate and nicotinamide metabolism

16
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  

KEGG

Biosynthesis of secondary metabolites

326
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15610443 NP_217824.1 Run
GO:0004731

purine-nucleoside phosphorylase activity

purine-nucleoside phosphorylase activity

Details: 
Catalysis of the reaction: purine nucleoside + phosphate = purine + alpha-D-ribose 1-phosphate.
GO Category: 
molecular_function
2
Total items in this category:  
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.210000 0.82

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: