Rv3308 Phosphomannomutase (EC 5.4.2.8)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv3308 pmmB Phosphomannomutase (EC 5.4.2.8) CDS 3694864 3696468 + 1 605 534 FALSE

Rv3308 (Phosphomannomutase (EC 5.4.2.8)) is predicted to be co-regulated in modules bicluster_0112 with residual 0.53 and bicluster_0533 with residual 0.51.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.70 and 3.00 for bicluster_0112 and 12.00 and 60.00 for bicluster_0533 respectively.

These modules are enriched for following go terms: phosphate-containing compound metabolic ..., phosphorus metabolic process, organophosphate biosynthetic process, coenzyme biosynthetic process, cofactor biosynthetic process.

This gene is found to be non-essential for growth on cholesterol.

Mutant available?:

Displaying 1 - 1 of 1
Gene Target Differential Expression Distance Expression pvalue Type
No results were found
Displaying 1 - 1 of 1
ChipSeq TF Differential Expression Distance Expression pvalue Type
No results were found
Motif 1 Motif 2 Residual
bicluster_0112
e.value: 
0.7
Motif Bicluster: 
e.value: 
3
Motif Bicluster: 
0.53
bicluster_0533
e.value: 
12
Motif Bicluster: 
e.value: 
60
Motif Bicluster: 
0.51
Product (LegacyBRC) Product (RefSeq)
PROBABLE PHOSPHOMANNOMUTASE PMMB [PHOSPHOMANNOSE MUTASE] phosphomannomutase
Operon # Operon
2160 Rv3307 - Rv3308
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Fructose and mannose metabolism

18
Total items in this category:  

KEGG

Amino sugar and nucleotide sugar metabolism

28
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  

KEGG

Biosynthesis of secondary metabolites

326
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15610444 NP_217825.1 Run
GO:0004615

phosphomannomutase activity

phosphomannomutase activity

Details: 
Catalysis of the reaction: alpha-D-mannose 1-phosphate = D-mannose 6-phosphate.
GO Category: 
molecular_function
2
Total items in this category:  
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
Quantitative Proteomics Data
Cholesterol Essentiality t-test p-value Cholesterol/Glycerol Ratio
non-essential 0.800000 1.18

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.