Rv3322c Possible methyltransferase (EC 2.1.1.-)

Summary
Product Feature Type Start End Strand Length AA Length is TF
Rv3322c Possible methyltransferase (EC 2.1.1.-) CDS 3708438 3709052 - 615 204 FALSE

Rv3322c (Possible methyltransferase (EC 2.1.1.-)) is predicted to be co-regulated in modules bicluster_0204 with residual 0.42 and bicluster_0561 with residual 0.52.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.00 and 6.20 for bicluster_0204 and 0.03 and 9.50 for bicluster_0561 respectively.

These modules are enriched for following go terms: Mo-molybdopterin cofactor biosynthetic p..., Mo-molybdopterin cofactor metabolic proc..., molybdopterin cofactor biosynthetic proc..., molybdopterin cofactor metabolic process, prosthetic group metabolic process monovalent inorganic cation transport, metal ion transport, cation-transporting ATPase activity, metal ion transmembrane transporter acti..., monovalent inorganic cation transmembran..., ATPase activity, coupled to transmembran....

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

Last update: 10/16/2017 - 15:27
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-14901 MT3423 200
Displaying 1 - 1 of 1
Gene Target Differential Expression Distance Expression pvalue Type
No results were found
Displaying 1 - 1 of 1
ChipSeq TF Differential Expression Distance Expression pvalue Type
No results were found
Motif 1 Motif 2 Residual
bicluster_0204
e.value: 
0.0000000019
Motif Bicluster: 
e.value: 
6.2
Motif Bicluster: 
0.42
bicluster_0561
e.value: 
0.027
Motif Bicluster: 
e.value: 
9.5
Motif Bicluster: 
0.52
Product (LegacyBRC) Product (RefSeq)
POSSIBLE METHYLTRANSFERASE methyltransferase
Operon # Operon
2169 - -
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Histidine metabolism

40
Total items in this category:  

KEGG

Tyrosine metabolism

41
Total items in this category:  

KEGG

Selenocompound metabolism

40
Total items in this category:  

KEGG

Polycyclic aromatic hydrocarbon degradation

47
Total items in this category:  

KEGG

Microbial metabolism in diverse environments

296
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
57117089 YP_177958.1 Run
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.890000 1.71

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: