Rv3332 N-acetylglucosamine-6-phosphate deacetylase (EC 3.5.1.25)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv3332 nagA N-acetylglucosamine-6-phosphate deacetylase (EC 3.5.1.25) CDS 3718595 3719746 + 1 152 383 FALSE

Rv3332 (N-acetylglucosamine-6-phosphate deacetylase (EC 3.5.1.25)) is predicted to be co-regulated in modules bicluster_0024 with residual 0.54 and bicluster_0379 with residual 0.53.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 1.30 and 1.50 for bicluster_0024 and 8.60 and 9.10 for bicluster_0379 respectively.

These modules are enriched for following go terms: NAD(P)+ transhydrogenase activity, oxidoreductase activity, acting on NAD(P....

This gene is found to be non-essential for growth on cholesterol.

Mutant available?:

Displaying 1 - 1 of 1
Gene Target Differential Expression Distance Expression pvalue Type
No results were found
Displaying 1 - 1 of 1
ChipSeq TF Differential Expression Distance Expression pvalue Type
No results were found
Motif 1 Motif 2 Residual
bicluster_0024
e.value: 
1.3
Motif Bicluster: 
e.value: 
1.5
Motif Bicluster: 
0.54
bicluster_0379
e.value: 
8.6
Motif Bicluster: 
e.value: 
9.1
Motif Bicluster: 
0.53
Product (LegacyBRC) Product (RefSeq)
PROBABLE N-ACETYLGLUCOSAMINE-6-PHOSPHATE DEACETYLASE NAGA [GLCNAC 6-P DEACETYLASE] N-acetylglucosamine-6-phosphate deacetylase
Operon # Operon
2176 Rv3331 - Rv3332
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Amino sugar and nucleotide sugar metabolism

28
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15610468 NP_217849.1 Run
GO:0008448

N-acetylglucosamine-6-phosphate deacetylase activity

N-acetylglucosamine-6-phosphate deacetylase activity

Details: 
Catalysis of the reaction: N-acetyl-D-glucosamine 6-phosphate + H2O = D-glucosamine 6-phosphate + acetate.
GO Category: 
molecular_function
1
Total items in this category:  
GO:0040007

growth

growth

Details: 
The increase in size or mass of an entire organism, a part of an organism or a cell.
GO Category: 
biological_process
621
Total items in this category:  
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
Quantitative Proteomics Data
Cholesterol Essentiality t-test p-value Cholesterol/Glycerol Ratio
non-essential 0.950000 2.06

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.